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Thus, a greater understanding of the interaction between CLL and T-cells could improve their utility. In this study, using two distinct xenograft models, we investigated whether xenografts recapitulate CLL biology including natural environmental interactions with B-cell receptors and T-cells and whether manipulation of autologous T-cells can expand the duration of CLL engraftment. We observed that primary CLL xenografts recapitulated both the tumour phenotype and T-cell repertoire observed in patients and that engraftment was significantly shorter for progressive tumours. Reduction of patients\u2019 T-cells to 2-5% of the initial T-cell number or specific depletion of CD8+ cells extended the limited xenograft duration of progressive cases to that characteristic of indolent disease. We conclude that manipulation of T-cells can enhance current CLL xenograft models expanding their utility for investigation of tumour biology and pre-clinical drug assessment.<\/jats:p>","DOI":"10.1242\/dmm.021147","type":"journal-article","created":{"date-parts":[[2015,8,21]],"date-time":"2015-08-21T00:59:25Z","timestamp":1440118765000},"source":"Crossref","is-referenced-by-count":2,"title":["T-cell number and subtype influence the disease course of primary chronic lymphocytic leukaemia xenografts in alymphoid mice"],"prefix":"10.1242","author":[{"given":"Ceri E.","family":"Oldreive","sequence":"first","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Skowronska","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Davies","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Parry","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo","family":"Agathanggelou","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Krysov","sequence":"additional","affiliation":[{"name":"CRUK Centre, Cancer Sciences Unit, University of Southampton, Southampton, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham","family":"Packham","sequence":"additional","affiliation":[{"name":"CRUK Centre, Cancer Sciences Unit, University of Southampton, Southampton, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew","family":"Rudzki","sequence":"additional","affiliation":[{"name":"Department of Pathology, Heart of England Hospital, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Cronin","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Vrzalikova","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Murray","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Odintsova","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Pratt","sequence":"additional","affiliation":[{"name":"School of Cancer Sciences, University of Birmingham, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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