{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T03:26:32Z","timestamp":1783740392754,"version":"3.55.0"},"reference-count":28,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":1463,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03V0556"],"award-info":[{"award-number":["03V0556"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03V0558"],"award-info":[{"award-number":["03V0558"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Animal models reflective of ulcerative colitis (UC) remain a major challenge, and yet are crucial to understand mechanisms underlying the onset of disease and inflammatory characteristics of relapses and remission. Mouse models in which colitis-like symptoms are induced through challenge with toxins such as oxazolone, dextran sodium sulfate (DSS) or 2,4,6-trinitrobenzenesulfonic acid (TNBS) have been instrumental in understanding the inflammatory processes of UC. However, these neither reflect the heterogeneous symptoms observed in the UC-affected population nor can they be used to test the efficacy of inhibitors developed against human targets where high sequence and structural similarity of the respective ligands is lacking. In an attempt to overcome these problems, we have developed a mouse model that relies on NOD-scid IL2R \u03b3null mice reconstituted with peripheral blood mononuclear cells derived from UC-affected individuals. Upon challenge with ethanol, mice developed colitis-like symptoms and changes in the colon architecture, characterized by influx of inflammatory cells, edema, crypt loss, crypt abscesses and epithelial hyperplasia, as previously observed in immune-competent mice. TARC, TGF\u03b21 and HGF expression increased in distal parts of the colon. Analysis of human leucocytes isolated from mouse spleen revealed an increase in frequencies of CD1a+, CD64+, CD163+ and TSLPR+ CD14+ monocytes, and antigen-experienced CD44+ CD4+ and CD8+ T-cells in response to ethanol. Analysis of human leucocytes from the colon of challenged mice identified CD14+ monocytes and CD11b+ monocytes as the predominant populations. Quantitative real-time PCR (RT-PCR) analysis from distal parts of the colon indicated that IFN\u03b3 might be one of the cytokines driving inflammation. Treatment with infliximab ameliorated symptoms and pathological manifestations, whereas pitrakinra had no therapeutic benefit. Thus, this model is partially reflective of the human disease and might help to increase the translation of animal and clinical studies.<\/jats:p>","DOI":"10.1242\/dmm.025452","type":"journal-article","created":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T01:03:38Z","timestamp":1470359018000},"page":"985-997","source":"Crossref","is-referenced-by-count":20,"title":["A mouse model for ulcerative colitis based on NOD-<i>scid<\/i> IL2R \u03b3null mice reconstituted with peripheral blood mononuclear cells from affected individuals"],"prefix":"10.1242","volume":"9","author":[{"given":"Pia","family":"Palamides","sequence":"first","affiliation":[{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich 1 , Munich 81377 , Germany"},{"name":"Gene Center, LMU Munich 2 Laboratory for Functional Genome Analysis (LAFUGA) , , Munich 81377 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrika","family":"Jodeleit","sequence":"additional","affiliation":[{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich 1 , Munich 81377 , Germany"},{"name":"Gene Center, LMU Munich 2 Laboratory for Functional Genome Analysis (LAFUGA) , , Munich 81377 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"F\u00f6hlinger","sequence":"additional","affiliation":[{"name":"Hospital of the University of Munich 3 Department of General- Visceral-, and Transplantation Surgery , , Nussbaumstr. 20, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Beigel","sequence":"additional","affiliation":[{"name":"Ludwig Maximilians University 4 Department of Medicine II-Grosshadern , , Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadja","family":"Herbach","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology 5 , Ludwig Maximilians University, Munich , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Mueller","sequence":"additional","affiliation":[{"name":"Julius von Sachs Institute, University of W\u00fcrzburg 6 , W\u00fcrzburg 97082 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich 1 , Munich 81377 , Germany"},{"name":"Gene Center, LMU Munich 2 Laboratory for Functional Genome Analysis (LAFUGA) , , Munich 81377 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Siebeck","sequence":"additional","affiliation":[{"name":"Hospital of the University of Munich 3 Department of General- Visceral-, and Transplantation Surgery , , Nussbaumstr. 20, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4756-261X","authenticated-orcid":false,"given":"Roswitha","family":"Gropp","sequence":"additional","affiliation":[{"name":"Hospital of the University of Munich 3 Department of General- Visceral-, and Transplantation Surgery , , Nussbaumstr. 20, Munich 80336 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061302532180700_DMM025452C1","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/s12016-012-8349-4","article-title":"Presence of anti-proteinase 3 antineutrophil cytoplasmic antibodies (anti-PR3 ANCA) as serologic markers in inflammatory bowel disease","volume":"45","author":"Arias-Loste","year":"2013","journal-title":"Clin. 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