{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:53:17Z","timestamp":1760550797494,"version":"3.41.2"},"reference-count":45,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":868,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31271574"],"award-info":[{"award-number":["31271574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2016A030310378"],"award-info":[{"award-number":["2016A030310378"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Benzene exposure is associated with various hematological disorders, especially leukemia. The reactive metabolite of benzene, 1,4-Benzoquinone (BQ), generated in bone marrow (BM), is suggested to be a key molecule in mediating benzene-induced hematotoxicity and carcinogenicity. Yet, its pathogenic role remains largely unknown due to lack of suitable vertebrate whole-organism models. Here, we present an in vivo study to reveal the effect of BQ exposure on hematotoxicity in zebrafish. From embryonic stages to adulthood, BQ exposure suppressed erythroid and lymphoid hematopoiesis but abnormally accumulated myeloid cells and precursors, which resembles benzene-induced cytopenia and myeloid dysplasia in humans. This myeloid expansion is caused by granulocyte but not macrophage lineage, emphasizing the significant role of lineage specificity in BQ-mediated hematopoietic toxicity. Analysis of the c-myb-deficient mutant cmybhkz3 revealed that BQ induced neutrophilia in a c-myb-dependent manner, demonstrating that c-myb is a key intrinsic mediator of BQ hematotoxicity. Our study reveals that BQ causes lineage-specific hematotoxicity in zebrafish from embryonic stages to adulthood. Since c-myb is indispensable for BQ to induce neutrophilia, c-myb may serve as a potential drug target for reversing BQ hematotoxicity.<\/jats:p>","DOI":"10.1242\/dmm.037903","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T15:47:57Z","timestamp":1553010477000},"source":"Crossref","is-referenced-by-count":7,"title":["Establishment of a zebrafish hematological disease model induced by 1,4-benzoquinone"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0045-4777","authenticated-orcid":false,"given":"Ao","family":"Zhang","sequence":"first","affiliation":[{"name":"Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, P.R. China"},{"name":"Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6021-6554","authenticated-orcid":false,"given":"Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7061-5495","authenticated-orcid":false,"given":"Junliang","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2812-9300","authenticated-orcid":false,"given":"Ning","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, P.R. 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