{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:03:39Z","timestamp":1772168619012,"version":"3.50.1"},"reference-count":33,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":223,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100012041","name":"Versus Arthritis","doi-asserted-by":"publisher","award":["20717"],"award-info":[{"award-number":["20717"]}],"id":[{"id":"10.13039\/501100012041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012041","name":"Versus Arthritis","doi-asserted-by":"publisher","award":["20859"],"award-info":[{"award-number":["20859"]}],"id":[{"id":"10.13039\/501100012041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012041","name":"Versus Arthritis","doi-asserted-by":"publisher","award":["MR\/R502182\/1"],"award-info":[{"award-number":["MR\/R502182\/1"]}],"id":[{"id":"10.13039\/501100012041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007155","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>CCN2 is a matricellular protein involved in several crucial biological processes. In particular, CCN2 is involved in cartilage development and in osteoarthritis. Ccn2 null mice exhibit a range of skeletal dysmorphisms, highlighting its importance in regulating matrix formation during development; however, its role in adult cartilage remains unclear. The aim of this study was to determine the role of CCN2 in postnatal chondrocytes in models of post-traumatic osteoarthritis (PTOA). Ccn2 deletion was induced in articular chondrocytes of male transgenic mice at 8\u2005weeks of age. PTOA was induced in knees either surgically or non-invasively by repetitive mechanical loading at 10\u2005weeks of age. Knee joints were harvested, scanned with micro-computed tomography and processed for histology. Sections were stained with Toluidine Blue and scored using the Osteoarthritis Research Society International (OARSI) grading system. In the non-invasive model, cartilage lesions were present in the lateral femur, but no significant differences were observed between wild-type (WT) and Ccn2 knockout (KO) mice 6\u2005weeks post-loading. In the surgical model, severe cartilage degeneration was observed in the medial compartments, but no significant differences were observed between WT and Ccn2 KO mice at 2, 4 and 8\u2005weeks post-surgery. We conclude that Ccn2 deletion in chondrocytes does not modify the development of PTOA in mice, suggesting that chondrocyte expression of CCN2 in adults is not a crucial factor in protecting cartilage from the degeneration associated with PTOA.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/dmm.044719","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T09:12:21Z","timestamp":1593681141000},"source":"Crossref","is-referenced-by-count":6,"title":["Post-traumatic osteoarthritis development is not modified by postnatal chondrocyte deletion of\n                    <i>Ccn2<\/i>"],"prefix":"10.1242","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8782-8016","authenticated-orcid":false,"given":"Craig M.","family":"Keenan","sequence":"first","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Ramos-Mucci","sequence":"additional","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6410-1482","authenticated-orcid":false,"given":"Ioannis","family":"Kanakis","sequence":"additional","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5293-2438","authenticated-orcid":false,"given":"Peter I.","family":"Milner","sequence":"additional","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Leask","sequence":"additional","affiliation":[{"name":"College of Dentistry, University of Saskatchewan 2 , Saskatoon, SK S7N 5E4 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-6057","authenticated-orcid":false,"given":"David","family":"Abraham","sequence":"additional","affiliation":[{"name":"Centre for Rheumatology and Connective Tissue Diseases, University College London 3 , London NW3 2PF , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Bou-Gharios","sequence":"additional","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9224-5253","authenticated-orcid":false,"given":"Blandine","family":"Poulet","sequence":"additional","affiliation":[{"name":"Institute of Life Course and Medical Sciences, University of Liverpool 1 Department of Musculoskeletal and Ageing Science , , William Henry Duncan Building , West Derby Street, Liverpool L7 8TX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"2024061302423908600_DMM044719C1","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nrrheum.2013.109","article-title":"Mesenchymal stem cells in joint disease and repair","volume":"9","author":"Barry","year":"2013","journal-title":"Nat. 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