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Mutation of genes encoding the ligand EDA, its transmembrane receptor EDAR and the intracellular signal transducer EDARADD leads to hypohidrotic ectodermal dysplasia, characterised by impaired development of teeth and hair, as well as cutaneous glands. The rodent ear canal has a large auditory sebaceous gland, the Zymbal\u2019s gland, the function of which in the health of the ear canal has not been determined. We report that EDA-deficient mice, EDAR-deficient mice and EDARADD-deficient rats have Zymbal\u2019s gland hypoplasia. EdaTa mice have 25% prevalence of otitis externa at postnatal day 21 and treatment with agonist anti-EDAR antibodies rescues Zymbal\u2019s glands. The aetiopathogenesis of otitis externa involves infection with Gram-positive cocci, and dosing pregnant and lactating EdaTa females and pups with enrofloxacin reduces the prevalence of otitis externa. We infer that the deficit of sebum is the principal factor in predisposition to bacterial infection, and the EdaTa mouse is a potentially useful microbial challenge model for human acute otitis externa.<\/jats:p>","DOI":"10.1242\/dmm.049034","type":"journal-article","created":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T11:01:14Z","timestamp":1643799674000},"source":"Crossref","is-referenced-by-count":2,"title":["The EDA-deficient mouse has Zymbal's gland hypoplasia and acute otitis externa"],"prefix":"10.1242","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-1228","authenticated-orcid":false,"given":"Jorge","family":"del-Pozo","sequence":"first","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies, University of Edinburgh 1 Veterinary Pathology , , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0452-4480","authenticated-orcid":false,"given":"Denis J.","family":"Headon","sequence":"additional","affiliation":[{"name":"Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh 2 , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James D.","family":"Glover","sequence":"additional","affiliation":[{"name":"Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh 2 , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Azar","sequence":"additional","affiliation":[{"name":"Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh 2 , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Schuepbach-Mallepell","sequence":"additional","affiliation":[{"name":"University of Lausanne 3 Department of Biochemistry , , Boveresses 155, CH-1066 Epalinges , Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4688-1670","authenticated-orcid":false,"given":"Mahmood F.","family":"Bhutta","sequence":"additional","affiliation":[{"name":"Royal Sussex County Hospital 4 Department of ENT , , Brighton BN2 5BE , UK"},{"name":"Brighton and Sussex Medical School 5 , Falmer, Brighton BN1 9PX , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Riddell","sequence":"additional","affiliation":[{"name":"Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh 2 , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Maxwell","sequence":"additional","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies, University of Edinburgh 1 Veterinary Pathology , , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4418-7746","authenticated-orcid":false,"given":"Elspeth","family":"Milne","sequence":"additional","affiliation":[{"name":"The Royal (Dick) School of Veterinary Studies, University of Edinburgh 1 Veterinary Pathology , , Edinburgh EH25 9RG , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0677-9409","authenticated-orcid":false,"given":"Pascal","family":"Schneider","sequence":"additional","affiliation":[{"name":"University of Lausanne 3 Department of Biochemistry , , Boveresses 155, CH-1066 Epalinges , Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5968-0176","authenticated-orcid":false,"given":"Michael","family":"Cheeseman","sequence":"additional","affiliation":[{"name":"Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh 2 , Edinburgh EH25 9RG , UK"},{"name":"University of Edinburgh, Institute of Genetics & Molecular Medicine 6 Division of Pathology , , Crewe Road, Edinburgh EH4 2XR , UK"},{"name":"Centre for Comparative Pathology 7 , Division of Pathology , , Crewe Road, Edinburgh EH4 2XR , UK"},{"name":"University of Edinburgh, Institute of Genetics & Molecular Medicine 7 , Division of Pathology , , Crewe Road, Edinburgh EH4 2XR , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"2024061306482555000_DMM049034C1","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1111\/joa.12344","article-title":"The development of the mammalian outer and middle ear","volume":"228","author":"Anthwal","year":"2016","journal-title":"J. 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