{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T10:41:47Z","timestamp":1792233707785,"version":"4.4.0"},"reference-count":31,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"crossref","award":["EMR\/2017\/001490"],"award-info":[{"award-number":["EMR\/2017\/001490"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"crossref","award":["3\/1\/3\/JRF-2014\/HRD-98 (31555)"],"award-info":[{"award-number":["3\/1\/3\/JRF-2014\/HRD-98 (31555)"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"crossref","award":["BT\/PR5480\/INF\/22\/158\/2012"],"award-info":[{"award-number":["BT\/PR5480\/INF\/22\/158\/2012"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"crossref","award":["BT.MED-II\/ATPC\/BSC\/01\/2010"],"award-info":[{"award-number":["BT.MED-II\/ATPC\/BSC\/01\/2010"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A mouse-adapted isolate of Japanese encephalitis virus (JEV), designated as JEV-S3, was generated by serially passaging the P20778 strain of the virus in 3- to 4-week-old C57BL\/6 mice. Blood-brain barrier leakage was evident in JEV-S3-infected mice, in which viral antigens and RNA were consistently demonstrated in the brain, along with infiltration of activated immune cells, as evidenced by an increased CD45+CD11b+ cell population. Histopathology studies showed the presence of perivascular cuffing, haemorrhage and necrotic foci in the virus-infected brain, conforming to the pathological changes seen in the brain of JEV-infected patients. Mass spectrometry studies characterized the molecular events leading to brain inflammation in the infected mice. Notably, a significant induction of inflammatory cytokines, such as IFN\u03b3, IL6, TNF\u03b1 and TGF\u03b2, was observed. Further, genome sequencing of the JEV-S3 isolate identified the mutations selected during the mouse passage of the virus. Overall, we present an in-depth characterization of a robust and reproducible mouse model of JEV infection. The JEV-S3 isolate will be a useful tool to screen antivirals and study virus pathogenesis in the adolescent mouse model.<\/jats:p>","DOI":"10.1242\/dmm.049176","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T04:54:47Z","timestamp":1630040087000},"source":"Crossref","is-referenced-by-count":34,"title":["Development and characterization of an animal model of Japanese encephalitis virus infection in adolescent C57BL\/6 mouse"],"prefix":"10.1242","volume":"14","author":[{"given":"Aarti","family":"Tripathi","sequence":"first","affiliation":[{"name":"Infection and Immunology, Translational Health Science and Technology Institute, Faridabad 121001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0600-1763","authenticated-orcid":false,"given":"Arup","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Infection and Immunology, Translational Health Science and Technology Institute, Faridabad 121001, India"},{"name":"Laboratory of Virology, Regional Centre for Biotechnology, Faridabad 121001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9984-3897","authenticated-orcid":false,"given":"Sudhanshu","family":"Vrati","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Regional Centre for Biotechnology, Faridabad 121001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"key":"2021111113114980100_DMM049176C1","doi-asserted-by":"publisher","first-page":"613","DOI":"10.3851\/IMP3180","article-title":"In vitro susceptibility of geographically and temporally distinct Zika viruses to favipiravir and ribavirin","volume":"22","author":"Baz","year":"2017","journal-title":"Antivir. 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