{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:33:09Z","timestamp":1760866389006,"version":"3.41.2"},"reference-count":31,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81970845","82000906"],"award-info":[{"award-number":["81970845","82000906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Sailing Program","award":["19YF1439200"],"award-info":[{"award-number":["19YF1439200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Bietti crystalline dystrophy (BCD) is an autosomal recessive inherited retinal disease, resulting in blindness in most patients. The etiology and development mechanism of it remain unclear. Given the defects in previous mouse models of BCD, we generated a new Cyp4v3\u2212\/\u2212 mouse model, using CRISPR\/Cas9 technology, for investigating the pathogenesis of BCD. We estimated the ocular phenotypes by fundus imaging, optical coherence tomography (OCT) and full-field scotopic electroretinography, and investigated the histological features by Hematoxylin and Eosin staining, Oil Red O staining and immunofluorescence. This model effectively exhibited age-related progression that mimicked the human ocular phenotypes. Moreover, gas chromatography-mass spectrometry and RNA-seq analysis indicated that the defect of Cyp4v3 led to the abnormal lipid metabolism, inflammation activation and oxidative stress of retina. Notably, inflammation activation and oxidative stress could also promote the progression of BCD in light-induced retinal degeneration. In conclusion, our data provided evidence that we established a novel and more effective Cyp4v3 knockout preclinical mouse model for BCD, which served as a useful tool for evaluating the effect of drugs and gene therapy in vivo.<\/jats:p>","DOI":"10.1242\/dmm.049222","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T14:26:52Z","timestamp":1646144812000},"source":"Crossref","is-referenced-by-count":8,"title":["A novel and efficient murine model of Bietti crystalline dystrophy"],"prefix":"10.1242","volume":"15","author":[{"given":"Yafang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, China200080"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai General 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Photomedicine, 100 Haining Road, Shanghai, China200080"},{"name":"National Clinical Research Center for Eye Diseases, 100 Haining Road, Shanghai, China200080"},{"name":"Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, 100 Haining Road, Shanghai, China200080"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4583-8686","authenticated-orcid":false,"given":"Xiaoling","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, China200080"},{"name":"Shanghai Key Laboratory of Ocular Fundus Diseases, 100 Haining Road, Shanghai, 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