{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:44:29Z","timestamp":1779893069318,"version":"3.53.1"},"reference-count":43,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"crossref","award":["18-04483S"],"award-info":[{"award-number":["18-04483S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["RTI2018-099250-B-100"],"award-info":[{"award-number":["RTI2018-099250-B-100"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-\u03b3 coactivator-1\u03b2 (PGC-1\u03b2; officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue; BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1\u03b2 knockout mice (PGC-1\u03b2-AT-KO mice) we aimed to learn whether specific PGC-1\u03b2 ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30\u00b0C) mutant mice were relatively sensitive to acute cold exposure (6\u00b0C). When these mice were subjected to cold exposure for 7\u2005days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1\u03b2-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1\u03b2-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1\u03b2 in controlling BAT lipid metabolism and thermogenesis.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/dmm.049223","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T09:01:42Z","timestamp":1650877302000},"source":"Crossref","is-referenced-by-count":10,"title":["Adipose tissue-specific ablation of PGC-1\u03b2 impairs thermogenesis in brown fat"],"prefix":"10.1242","volume":"15","author":[{"given":"Ji\u0159\u00ed","family":"Funda","sequence":"first","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"},{"name":"Department of Physiology, Faculty of Science, Charles University in Prague, 128 44 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep A.","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism and Obesity, Vall d'Hebron-Institut de Recerca, Universitat Aut\u00f2noma de Barcelona, 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas, Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Bardova","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Adamcova","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Illaria","family":"Irodenko","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Flachs","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Jedlickova","sequence":"additional","affiliation":[{"name":"Research Unit for Rare Diseases, Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliska","family":"Haasova","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"},{"name":"Department of Physiology, Faculty of Science, Charles University in Prague, 128 44 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Rossmeisl","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Kopecky","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6154-2175","authenticated-orcid":false,"given":"Petra","family":"Janovska","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"2022042509013096900_DMM049223C1","doi-asserted-by":"publisher","first-page":"3737","DOI":"10.3390\/nu12123737","article-title":"Additive effects of omega-3 fatty acids and thiazolidinediones in mice fed a high-fat diet: triacylglycerol\/fatty acid cycling in adipose tissue","volume":"12","author":"Bardova","year":"2020","journal-title":"Nutrients"},{"key":"2022042509013096900_DMM049223C2","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/B978-0-444-63912-7.00010-2","article-title":"Shivering and nonshivering thermogenesis in skeletal muscles","volume":"156","author":"Blondin","year":"2018","journal-title":"Handb. 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