{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:03:01Z","timestamp":1790758981397,"version":"4.1.0"},"reference-count":32,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T00:00:00Z","timestamp":1666569600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Adherent-invasive Escherichia coli (AIEC) were investigated for their involvement in the induction\/chronicity of intestinal inflammation in Crohn's disease (CD). AIEC gut establishment is favoured by overexpression of the glycoprotein CEACAM6 in the ileal epithelium. We generated a transgenic mouse model, named \u2018Vill-hCC6\u2019, in which the human CEACAM6 gene was under the control of the villin promoter, conditioning expression in the small intestine. We demonstrated that CEACAM6 is strongly expressed in the small intestine mucosa and is correlated with numerous glycosylations displayed at the brush border of enterocytes. Ex vivo, the AIEC\u2013enterocyte interaction was enhanced by CEACAM6 expression and necessitated the presence of the bacterial adhesive factor FimH. Finally, AIEC bacteria preferentially persisted in a FimH-dependent manner in the ileal mucosa of Vill-hCC6 mice compared to wild-type mice. This preclinical model opens new perspectives in the mechanistic study of the AIEC pathobiont and represents a valuable tool to evaluate the efficacy of new strategies to eliminate AIEC implanted in the ileal mucosa, such as phages, inhibitory and\/or anti-virulence molecules, or CRISPR-based strategies targeting virulence or fitness factors of AIEC bacteria.<\/jats:p>","DOI":"10.1242\/dmm.049707","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T09:00:07Z","timestamp":1664442007000},"source":"Crossref","is-referenced-by-count":17,"title":["An adherent-invasive <i>Escherichia coli<\/i>-colonized mouse model to evaluate microbiota-targeting strategies in Crohn's disease"],"prefix":"10.1242","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2288-2959","authenticated-orcid":false,"given":"Adeline","family":"Sivignon","sequence":"first","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"},{"name":"Institut Universitaire de Technologie, G\u00e9nie Biologique 2 , Aubi\u00e8re 63172 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lissa","family":"Chervy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Chevarin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia","family":"Ragot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6066-4584","authenticated-orcid":false,"given":"Elisabeth","family":"Billard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"},{"name":"Institut Universitaire de Technologie, G\u00e9nie Biologique 2 , Aubi\u00e8re 63172 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0078-8996","authenticated-orcid":false,"given":"J\u00e9r\u00e9my","family":"Denizot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"},{"name":"Institut Universitaire de Technologie, G\u00e9nie Biologique 2 , Aubi\u00e8re 63172 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-7844","authenticated-orcid":false,"given":"Nicolas","family":"Barnich","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, Inserm U1071, INRAE USC 1382, Microbes, Intestin, Inflammation et Susceptibilit\u00e9 de l'H\u00f4te (M2iSH) 1 , Clermont-Ferrand 63001 , France"},{"name":"Institut Universitaire de Technologie, G\u00e9nie Biologique 2 , Aubi\u00e8re 63172 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,10,24]]},"reference":[{"key":"2022102413122208300_DMM049707C1","doi-asserted-by":"publisher","first-page":"567929","DOI":"10.1155\/2014\/567929","article-title":"Understanding host-adherent-invasive Escherichia coli interaction in Crohn's disease: opening up new therapeutic strategies","volume":"2014","author":"Agus","year":"2014","journal-title":"Biomed. 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