{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:01:57Z","timestamp":1765486917075,"version":"3.41.2"},"reference-count":58,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Gulf Coast Consortium"},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"crossref","award":["R01DE011931"],"award-info":[{"award-number":["R01DE011931"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"crossref","award":["RR14007"],"award-info":[{"award-number":["RR14007"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01GM124043"],"award-info":[{"award-number":["R01GM124043"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Linda and Mark Quick Award for Basic Science"},{"DOI":"10.13039\/100007313","name":"University of Texas MD Anderson Cancer Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007313","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Facial development requires a complex and coordinated series of cellular events that, when perturbed, can lead to structural birth defects. A quantitative approach to quickly assess morphological changes could address how genetic or environmental inputs lead to differences in facial shape and promote malformations. Here, we report on a method to rapidly analyze craniofacial development in zebrafish embryos using facial analytics based on a coordinate extrapolation system, termed zFACE. Confocal images capture facial structures and morphometric data are quantified based on anatomical landmarks present during development. The quantitative morphometric data can detect phenotypic variation and inform on changes in facial morphology. We applied this approach to show that loss of smarca4a in developing zebrafish leads to craniofacial anomalies, microcephaly and alterations in brain morphology. These changes are characteristic of Coffin\u2013Siris syndrome, a rare human genetic disorder associated with mutations in SMARCA4. Multivariate analysis of zFACE data facilitated the classification of smarca4a mutants based on changes in specific phenotypic characteristics. Together, zFACE provides a way to rapidly and quantitatively assess the impact of genetic alterations on craniofacial development in zebrafish.<\/jats:p>","DOI":"10.1242\/dmm.049868","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T06:40:38Z","timestamp":1682577638000},"source":"Crossref","is-referenced-by-count":4,"title":["Facial analytics based on a coordinate extrapolation system (zFACE) for morphometric phenotyping of developing zebrafish"],"prefix":"10.1242","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7695-4319","authenticated-orcid":false,"given":"Lorena","family":"Maili","sequence":"first","affiliation":[{"name":"Pediatric Research Center 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"McGovern Medical School University of Texas Health Science Center-Houston 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences 2 Genetics and Epigenetics Graduate Program , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar E.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center 3 Department of Genetics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip H.","family":"Kahan","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center 3 Department of Genetics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frankie","family":"Chiu","sequence":"additional","affiliation":[{"name":"Pediatric Research Center 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"McGovern Medical School University of Texas Health Science Center-Houston 1 , Department of Pediatrics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen T.","family":"Larson","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center 3 Department of Genetics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8758-8254","authenticated-orcid":false,"given":"S. Shahrukh","family":"Hashmi","sequence":"additional","affiliation":[{"name":"Pediatric Research Center 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"McGovern Medical School University of Texas Health Science Center-Houston 1 , Department of Pediatrics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9087-3379","authenticated-orcid":false,"given":"Jacqueline T.","family":"Hecht","sequence":"additional","affiliation":[{"name":"Pediatric Research Center 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"McGovern Medical School University of Texas Health Science Center-Houston 1 , Department of Pediatrics , , Houston, TX 77030 , USA"},{"name":"The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences 2 Genetics and Epigenetics Graduate Program , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-6782","authenticated-orcid":false,"given":"George T.","family":"Eisenhoffer","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences 2 Genetics and Epigenetics Graduate Program , , Houston, TX 77030 , USA"},{"name":"The University of Texas MD Anderson Cancer Center 3 Department of Genetics , , Houston, TX 77030 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"2023060213543229700_DMM049868C1","doi-asserted-by":"publisher","first-page":"12792","DOI":"10.1073\/pnas.0403929101","article-title":"Identification of 315 genes essential for early zebrafish development","volume":"101","author":"Amsterdam","year":"2004","journal-title":"Proc. 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