{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:20:16Z","timestamp":1791872416650,"version":"4.3.4"},"reference-count":51,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"AFM-T\u00e9l\u00e9thon","award":["23551"],"award-info":[{"award-number":["23551"]}]},{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"crossref","award":["TMPGMFU22TT"],"award-info":[{"award-number":["TMPGMFU22TT"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005202","name":"Muscular Dystrophy Association","doi-asserted-by":"crossref","award":["MDA 968551"],"award-info":[{"award-number":["MDA 968551"]}],"id":[{"id":"10.13039\/100005202","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministero dell'Istruzione, dell'Universit\u00e0 e della Ricerca","award":["201742SBXA_004"],"award-info":[{"award-number":["201742SBXA_004"]}]},{"name":"Universita degli Studi di Roma Tor Vergata"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In human dystrophies, progressive muscle wasting is exacerbated by ectopic deposition of fat and fibrous tissue originating from fibro\/adipogenic progenitors (FAPs). In degenerating muscles, the ability of these cells to promote successful healing is attenuated, and FAPs aberrantly expand and differentiate into adipocytes and fibroblasts. Thus, arresting the fibro\/adipogenic fate of FAPs, without affecting their physiological role, represents a valuable therapeutic strategy for patients affected by muscle diseases. Here, using a panel of adipose progenitor cells, including human-derived FAPs, coupled with pharmacological perturbations and proteome profiling, we report that LY2090314 interferes with a genuine adipogenic program acting as WNT surrogate for the stabilization of a competent \u03b2-catenin transcriptional complex. To predict the beneficial impact of LY2090314 in limiting ectopic deposition of fat in human muscles, we combined a poly-ethylene-glycol-fibrinogen biomimetic matrix with these progenitor cells to create a miniaturized 3D model of adipogenesis. Using this scalable system, we demonstrated that a two-digit nanomolar dose of this compound effectively represses adipogenesis at higher 3D scale, thus indicating the potential for LY2090314 to limit FAP-derived fat infiltrates in dystrophic muscles.<\/jats:p>","DOI":"10.1242\/dmm.049915","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T09:55:07Z","timestamp":1685958907000},"source":"Crossref","is-referenced-by-count":14,"title":["A 3D adipogenesis platform to study the fate of fibro\/adipogenic progenitors in muscular dystrophies"],"prefix":"10.1242","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5333-7502","authenticated-orcid":false,"given":"Alessio","family":"Reggio","sequence":"first","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3613-2721","authenticated-orcid":false,"given":"Francesca","family":"De Paolis","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"},{"name":"University of Rome \u2018Tor Vergata\u2019 2 Cellular and Molecular Biology, Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3032-532X","authenticated-orcid":false,"given":"Salma","family":"Bousselmi","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3439-2498","authenticated-orcid":false,"given":"Felice","family":"Cicciarelli","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Bernardini","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8971-551X","authenticated-orcid":false,"given":"Alberto","family":"Rainer","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Campus Bio-Medico 3 Department of Engineering , , 00128 Rome , Italy"},{"name":"Institute of Nanotechnology (NANOTEC), National Research Council 4 , 73100 Lecce , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6964-8567","authenticated-orcid":false,"given":"Dror","family":"Seliktar","sequence":"additional","affiliation":[{"name":"Techion Institute 5 Department of Biomedical Engineering , , 32000 Haifa , Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5981-504X","authenticated-orcid":false,"given":"Stefano","family":"Testa","sequence":"additional","affiliation":[{"name":"Aix Marseille University, INSERM, Marseille Medical Genetics (MMG) 6 , 13005 Marseille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-7677","authenticated-orcid":false,"given":"Carmine","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine (TIGEM) 7 , 80078 Pozzuoli , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9942-9389","authenticated-orcid":false,"given":"Paolo","family":"Grumati","sequence":"additional","affiliation":[{"name":"Telethon Institute of Genetics and Medicine (TIGEM) 7 , 80078 Pozzuoli , Italy"},{"name":"Federico II University 8 Department of Clinical Medicine and Surgery , , 80138 Naples , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Cannata","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8372-305X","authenticated-orcid":false,"given":"Claudia","family":"Fuoco","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1742-3969","authenticated-orcid":false,"given":"Cesare","family":"Gargioli","sequence":"additional","affiliation":[{"name":"University of Rome \u2018Tor Vergata\u2019 1 Department of Biology , , 00133 Rome , 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