{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:26:13Z","timestamp":1785932773139,"version":"3.56.0"},"reference-count":60,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"crossref","award":["EP\/R512540\/1"],"award-info":[{"award-number":["EP\/R512540\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"crossref","award":["EP\/R00188X\/1"],"award-info":[{"award-number":["EP\/R00188X\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"crossref","award":["EP\/L015498\/1"],"award-info":[{"award-number":["EP\/L015498\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["OPP1181199"],"award-info":[{"award-number":["OPP1181199"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Wellcome","award":["100993\/Z\/13\/Z"],"award-info":[{"award-number":["100993\/Z\/13\/Z"]}]},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"crossref","award":["RD-08-2800"],"award-info":[{"award-number":["RD-08-2800"]}],"id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["213435\/Z\/18\/Z"],"award-info":[{"award-number":["213435\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001773","name":"University of New South Wales","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001773","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Phenotypic cell-based screens are critical tools for discovering candidate drugs for development, yet identification of the cellular target and mode of action of a candidate drug is often lacking. Using an imaging-based screen, we recently discovered an N-[(4-hydroxychroman-4-yl)methyl]-sulphonamide (N-4HCS) compound, DDD01035881, that blocks male gamete formation in the malaria parasite life cycle and subsequent transmission of the parasite to the mosquito with nanomolar activity. To identify the target(s) of DDD01035881, and of the N-4HCS class of compounds more broadly, we synthesised a photoactivatable derivative, probe 2. Photoaffinity labelling of probe 2 coupled with mass spectrometry identified the 16\u2005kDa Plasmodium falciparum parasitophorous vacuole membrane protein Pfs16 as a potential parasite target. Complementary methods including cellular thermal shift assays confirmed that the parent molecule DDD01035881 stabilised Pfs16 in lysates from activated mature gametocytes. Combined with high-resolution, fluorescence and electron microscopy data, which demonstrated that parasites inhibited with N-4HCS compounds phenocopy the targeted deletion of Pfs16 in gametocytes, these data implicate Pfs16 as a likely target of DDD01035881. This finding establishes N-4HCS compounds as being flexible and effective starting candidates from which transmission-blocking antimalarials can be developed in the future.<\/jats:p>","DOI":"10.1242\/dmm.049950","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T07:08:44Z","timestamp":1675062524000},"source":"Crossref","is-referenced-by-count":14,"title":["A novel class of sulphonamides potently block malaria transmission by targeting a\n                    <i>Plasmodium<\/i>\n                    vacuole membrane protein"],"prefix":"10.1242","volume":"16","author":[{"given":"Sabrina","family":"Yahiya","sequence":"first","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlie N.","family":"Saunders","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"},{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hassan","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Straschil","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver J.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainoa","family":"Rueda-Zubiaurre","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Haase","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Vizcay-Barrena","sequence":"additional","affiliation":[{"name":"Centre for Ultrastructural Imaging, New Hunt's House, Guy's Campus, King's College London 3 , London SE1 1UL , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mufuliat Toyin","family":"Famodimu","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Jordan","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Delves","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2213-5814","authenticated-orcid":false,"given":"Edward W.","family":"Tate","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Barnard","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1767-7072","authenticated-orcid":false,"given":"Matthew J.","family":"Fuchter","sequence":"additional","affiliation":[{"name":"Imperial College London, Molecular Sciences Research Hub 2 Department of Chemistry , , White City Campus, Wood Lane, London W12 OBZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0275-352X","authenticated-orcid":false,"given":"Jake","family":"Baum","sequence":"additional","affiliation":[{"name":"Imperial College London 1 Department of Life Sciences , , Sir Alexander Fleming Building, Exhibition Road, South Kensington, London SW7 2AZ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"2023013012073954200_DMM049950C1","doi-asserted-by":"publisher","first-page":"3543","DOI":"10.1038\/s41598-018-21801-3","article-title":"Sequential membrane rupture and vesiculation during plasmodium berghei gametocyte egress from the red blood cell","volume":"8","author":"Andreadaki","year":"2018","journal-title":"Sci. Rep."},{"key":"2023013012073954200_DMM049950C2","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1186\/1475-2875-13-418","article-title":"Primaquine: the risks and the benefits","volume":"13","author":"Ashley","year":"2014","journal-title":"Malar. J."},{"key":"2023013012073954200_DMM049950C4","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1017\/S0031182000068219","article-title":"Subcellular localization of Pfs16, a Plasmodium falciparum gametocyte antigen","volume":"108","author":"Baker","year":"1994","journal-title":"Parasitology"},{"key":"2023013012073954200_DMM049950C5","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/s41467-017-00572-x","article-title":"A potent series targeting the malarial cGMP-dependent protein kinase clears infection and blocks transmission","volume":"8","author":"Baker","year":"2017","journal-title":"Nat. Commun."},{"key":"2023013012073954200_DMM049950C6","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1016\/j.pt.2016.04.017","article-title":"Discovering new transmission-blocking antimalarial compounds: challenges and opportunities","volume":"32","author":"Birkholtz","year":"2016","journal-title":"Trends Parasitol."},{"key":"2023013012073954200_DMM049950C7","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/j.pt.2022.01.011","article-title":"Transmission-blocking drugs for malaria elimination","volume":"38","author":"Birkholtz","year":"2022","journal-title":"Trends Parasitol."},{"key":"2023013012073954200_DMM049950C8","doi-asserted-by":"publisher","first-page":"1969","DOI":"10.1016\/S0140-6736(10)60549-1","article-title":"Global, regional, and national causes of child mortality in 2008: a systematic analysis","volume":"375","author":"Black","year":"2010","journal-title":"Lancet"},{"key":"2023013012073954200_DMM049950C9","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1038\/ncomms2840","article-title":"Transmission-blocking interventions eliminate malaria from laboratory populations","volume":"4","author":"Blagborough","year":"2013","journal-title":"Nat. Commun."},{"key":"2023013012073954200_DMM049950C10","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1038\/nprot.2015.072","article-title":"An assay to probe Plasmodium falciparum growth, transmission stage formation and early gametocyte development","volume":"10","author":"Brancucci","year":"2015","journal-title":"Nat. Protoc."},{"key":"2023013012073954200_DMM049950C11","doi-asserted-by":"publisher","first-page":"5948","DOI":"10.1002\/anie.201500342","article-title":"Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development","volume":"54","author":"Broncel","year":"2015","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"2023013012073954200_DMM049950C12","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/0166-6851(94)90111-2","article-title":"Cellular location and temporal expression of the Plasmodium falciparum sexual stage antigen Pfs16","volume":"65","author":"Bruce","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023013012073954200_DMM049950C13","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/s12936-016-1675-x","article-title":"New developments in anti-malarial target candidate and product profiles","volume":"16","author":"Burrows","year":"2017","journal-title":"Malar. J."},{"key":"2023013012073954200_DMM049950C14","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"2023013012073954200_DMM049950C61","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nmeth.2075","article-title":"Icy: an open bioimage informatics platform for extended reproducible research","volume":"9","author":"de Chaumont","year":"2012","journal-title":"Nat. Methods"},{"key":"2023013012073954200_DMM049950C15","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1111\/j.1365-2958.2007.06039.x","article-title":"The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite Plasmodium falciparum","volume":"67","author":"de Koning-Ward","year":"2008","journal-title":"Mol. Microbiol."},{"key":"2023013012073954200_DMM049950C16","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.molbiopara.2018.01.002","article-title":"Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites","volume":"220","author":"Deligianni","year":"2018","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023013012073954200_DMM049950C17","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.1038\/nprot.2016.096","article-title":"Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions","volume":"11","author":"Delves","year":"2016","journal-title":"Nat. Protoc."},{"key":"2023013012073954200_DMM049950C18","doi-asserted-by":"publisher","first-page":"3805","DOI":"10.1038\/s41467-018-05777-2","article-title":"A high throughput screen for next-generation leads targeting malaria parasite transmission","volume":"9","author":"Delves","year":"2018","journal-title":"Nat. Commun."},{"key":"2023013012073954200_DMM049950C20","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1038\/s41596-020-0310-z","article-title":"Cellular thermal shift assay for the identification of drug-target interactions in the Plasmodium falciparum proteome","volume":"15","author":"Dziekan","year":"2020","journal-title":"Nat. Protoc."},{"key":"2023013012073954200_DMM049950C21","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1128\/EC.00008-11","article-title":"Protein targeting to the parasitophorous vacuole membrane of Plasmodium falciparum","volume":"10","author":"Eksi","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"2023013012073954200_DMM049950C22","doi-asserted-by":"publisher","first-page":"e26524","DOI":"10.7554\/eLife.26524","article-title":"Multiple short windows of calcium-dependent protein kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis","volume":"6","author":"Fang","year":"2017","journal-title":"eLife"},{"key":"2023013012073954200_DMM049950C23","doi-asserted-by":"publisher","first-page":"398","DOI":"10.3390\/ijerph14040398","article-title":"Attractive Toxic Sugar Bait (ATSB) for control of mosquitoes and its impact on non-target organisms: a review","volume":"14","author":"Fiorenzano","year":"2017","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"2023013012073954200_DMM049950C24","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1016\/j.pt.2020.05.011","article-title":"When is a plasmodium-infected mosquito an infectious mosquito?","volume":"36","author":"Graumans","year":"2020","journal-title":"Trends Parasitol."},{"key":"2023013012073954200_DMM049950C25","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.1073\/pnas.1619441114","article-title":"Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress","volume":"114","author":"Hale","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2023013012073954200_DMM049950C26","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1111\/j.1476-5381.2010.01127.x","article-title":"Principles of early drug discovery","volume":"162","author":"Hughes","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"2023013012073954200_DMM049950C27","doi-asserted-by":"publisher","first-page":"111261","DOI":"10.1016\/j.molbiopara.2020.111261","article-title":"Observation of morphological changes of female osmiophilic bodies prior to Plasmodium gametocyte egress from erythrocytes","volume":"236","author":"Ishino","year":"2020","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023013012073954200_DMM049950C28","doi-asserted-by":"publisher","first-page":"2100","DOI":"10.1038\/nprot.2014.138","article-title":"The cellular thermal shift assay for evaluating drug target interactions in cells","volume":"9","author":"Jafari","year":"2014","journal-title":"Nat. Protoc."},{"key":"2023013012073954200_DMM049950C29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S0031182000081609","article-title":"DNA synthesis in gametocytes of Plasmodium falciparum","volume":"96","author":"Janse","year":"1988","journal-title":"Parasitology"},{"key":"2023013012073954200_DMM049950C30","doi-asserted-by":"publisher","first-page":"2602","DOI":"10.1021\/acs.chemrev.5b00346","article-title":"Degradable controlled-release polymers and polymeric nanoparticles: mechanisms of controlling drug release","volume":"116","author":"Kamaly","year":"2016","journal-title":"Chem. Rev."},{"key":"2023013012073954200_DMM049950C32","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.molbiopara.2003.10.014","article-title":"Functional analysis of Plasmodium falciparum parasitophorous vacuole membrane protein (Pfs16) during gametocytogenesis and gametogenesis by targeted gene disruption","volume":"133","author":"Kongkasuriyachai","year":"2004","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023013012073954200_DMM049950C33","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1038\/nmeth.2834","article-title":"Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells","volume":"11","author":"Kulak","year":"2014","journal-title":"Nat. Methods"},{"key":"2023013012073954200_DMM049950C34","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.exppara.2007.01.021","article-title":"Plasmodium falciparum: mRNA co-expression and protein co-localisation of two gene products upregulated in early gametocytes","volume":"116","author":"Lanfrancotti","year":"2007","journal-title":"Exp. Parasitol."},{"key":"2023013012073954200_DMM049950C35","doi-asserted-by":"publisher","first-page":"6087","DOI":"10.1093\/nar\/gkw536","article-title":"Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes: molecular insight into sex-specific processes and translational repression","volume":"44","author":"Lasonder","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"2023013012073954200_DMM049950C36","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1186\/1471-2164-12-587","article-title":"Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum","volume":"12","author":"L\u00f3pez-Barrag\u00e1n","year":"2011","journal-title":"BMC Genomics"},{"key":"2023013012073954200_DMM049950C37","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1021\/acsinfecdis.7b00276","article-title":"Using in vitro evolution and whole genome analysis to discover next generation targets for antimalarial drug discovery","volume":"4","author":"Luth","year":"2018","journal-title":"ACS Infect Dis."},{"key":"2023013012073954200_DMM049950C38","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1128\/EC.00336-09","article-title":"Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages","volume":"9","author":"MacKellar","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"2023013012073954200_DMM049950C39","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1126\/science.1233606","article-title":"Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay","volume":"341","author":"Martinez Molina","year":"2013","journal-title":"Science"},{"key":"2023013012073954200_DMM049950C40","article-title":"Pfs16, a potential vaccine candidate against the human malaria parasite Plasmodium falciparum","volume-title":"PhD thesis","author":"Moelans","year":"1995"},{"key":"2023013012073954200_DMM049950C41","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1093\/femsre\/fuz010","article-title":"Revisiting gametocyte biology in malaria parasites","volume-title":"FEMS Microbiol. Rev.","author":"Ngotho","year":"2019"},{"key":"2023013012073954200_DMM049950C42","doi-asserted-by":"publisher","first-page":"458","DOI":"10.3389\/fcimb.2021.654216","article-title":"Studies of the parasite-midgut interaction reveal plasmodium proteins important for malaria transmission to mosquitoes","volume":"11","author":"Niu","year":"2021","journal-title":"Front. Cell Infect. Microbiol."},{"key":"2023013012073954200_DMM049950C43","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1093\/infdis\/jit522","article-title":"A specific inhibitor of PfCDPK4 blocks malaria transmission: chemical-genetic validation","volume":"209","author":"Ojo","year":"2014","journal-title":"J. Infect. Dis."},{"key":"2023013012073954200_DMM049950C44","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1038\/s41586-019-0973-1","article-title":"Exposing Anopheles mosquitoes to antimalarials blocks Plasmodium parasite transmission","volume":"567","author":"Paton","year":"2019","journal-title":"Nature"},{"key":"2023013012073954200_DMM049950C45","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1038\/nrmicro1529","article-title":"The silent path to thousands of merozoites: the Plasmodium liver stage","volume":"4","author":"Prud\u00eancio","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"2023013012073954200_DMM049950C46","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/S0378-1119(03)00381-0","article-title":"The Plasmodium falciparum family of Rab GTPases","volume":"306","author":"Quevillon","year":"2003","journal-title":"Gene"},{"key":"2023013012073954200_DMM049950C47","doi-asserted-by":"publisher","first-page":"7292","DOI":"10.1128\/AAC.03666-14","article-title":"A male and female gametocyte functional viability assay to identify biologically relevant malaria transmission-blocking drugs","volume":"58","author":"Ruecker","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"2023013012073954200_DMM049950C48","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1021\/acs.jmedchem.9b00898","article-title":"Structure-activity relationship studies of a novel class of transmission blocking antimalarials targeting male gametes","volume":"63","author":"Rueda-Zubiaurre","year":"2019","journal-title":"J. Med. Chem."},{"key":"2023013012073954200_DMM049950C49","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S003118200005160X","article-title":"Gametocytogenesis of Plasmodium falciparum in vitro: an electron microscopic study","volume":"84","author":"Sinden","year":"1982","journal-title":"Parasitology"},{"key":"2023013012073954200_DMM049950C50","doi-asserted-by":"publisher","first-page":"e1006336","DOI":"10.1371\/journal.ppat.1006336","article-title":"Developing transmission-blocking strategies for malaria control","volume":"13","author":"Sinden","year":"2017","journal-title":"PLoS Pathog."},{"key":"2023013012073954200_DMM049950C51","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1098\/rspb.1978.0051","article-title":"Gametocyte and gamete development in Plasmodium falciparum","volume":"201","author":"Sinden","year":"1978","journal-title":"Proc. R. Soc. Lond. B Biol. Sci."},{"key":"2023013012073954200_DMM049950C52","doi-asserted-by":"publisher","first-page":"159","DOI":"10.4155\/fmc.14.152","article-title":"Photoaffinity labeling in target- and binding-site identification","volume":"7","author":"Smith","year":"2015","journal-title":"Future Med. Chem."},{"key":"2023013012073954200_DMM049950C62","doi-asserted-by":"publisher","first-page":"1735","DOI":"10.1109\/ISBI.2012.6235915","article-title":"Blind deconvolution of 3D data in wide field fluorescence microscopy","author":"Soulez","year":"2012","journal-title":"9th IEEE International Symposium on Biomedical Imaging (ISBI)"},{"key":"2023013012073954200_DMM049950C53","doi-asserted-by":"publisher","first-page":"610200","DOI":"10.3389\/fcimb.2020.610200","article-title":"Rab5b-associated Arf1 GTPase regulates export of N-Myristoylated Adenylate Kinase 2 from the endoplasmic reticulum in plasmodium falciparum","volume":"10","author":"Taku","year":"2020","journal-title":"Front. Cell Infect. Microbiol."},{"key":"2023013012073954200_DMM049950C54","doi-asserted-by":"publisher","first-page":"D506","DOI":"10.1093\/nar\/gky1049","article-title":"UniProt: a worldwide hub of protein knowledge","volume":"47","author":"UniProt Consortium","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2023013012073954200_DMM049950C63","doi-asserted-by":"publisher","first-page":"e175","DOI":"10.1016\/S2666-5247(21)00062-8","article-title":"Tafenoquine for the prevention of Plasmodium vivax malaria relapse","volume":"2","author":"Watson","year":"2021","journal-title":"Lancet Microbe"},{"key":"2023013012073954200_DMM049950C55","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1038\/nrd4573","article-title":"Malaria medicines: a glass half full?","volume":"14","author":"Wells","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"2023013012073954200_DMM049950C56","unstructured":"World Health Organization\n            \n           (2022). World Malaria Report. Geneva, Switzerland: World Health Organization."},{"key":"2023013012073954200_DMM049950C57","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.chembiol.2015.01.003","article-title":"Global analysis of protein N-myristoylation and exploration of N-myristoyltransferase as a drug target in the neglected human pathogen Leishmania donovani","volume":"22","author":"Wright","year":"2015","journal-title":"Chem. Biol."},{"key":"2023013012073954200_DMM049950C58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cbpa.2019.01.029","article-title":"The antimalarial screening landscape-looking beyond the asexual blood stage","volume":"50","author":"Yahiya","year":"2019","journal-title":"Curr. Opin. Chem. Biol."},{"key":"2023013012073954200_DMM049950C59","doi-asserted-by":"publisher","first-page":"e1010276","DOI":"10.1371\/journal.ppat.1010276","article-title":"Live-cell fluorescence imaging of microgametogenesis in the human malaria parasite Plasmodium falciparum","volume":"18","author":"Yahiya","year":"2022","journal-title":"PLoS Pathog."},{"key":"2023013012073954200_DMM049950C60","doi-asserted-by":"publisher","first-page":"eaap7847","DOI":"10.1126\/science.aap7847","article-title":"Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis","volume":"360","author":"Zhang","year":"2018","journal-title":"Science"}],"container-title":["Disease Models &amp; Mechanisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.049950\/2457539\/dmm049950.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.049950\/2457539\/dmm049950.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T07:08:54Z","timestamp":1675062534000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dmm\/article\/16\/2\/dmm049950\/286739\/A-novel-class-of-sulphonamides-potently-block"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,30]]},"references-count":60,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dmm.049950","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.06.14.448287","asserted-by":"object"}]},"ISSN":["1754-8403","1754-8411"],"issn-type":[{"value":"1754-8403","type":"print"},{"value":"1754-8411","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,30]]},"article-number":"dmm049950"}}