{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T01:54:15Z","timestamp":1783389255867,"version":"3.54.6"},"reference-count":59,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:00:00Z","timestamp":1683072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["105570\/Z\/14\/A"],"award-info":[{"award-number":["105570\/Z\/14\/A"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000858","name":"University of Sheffield","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000858","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000849","name":"National Centre for the Replacement, Refinement and Reduction of Animals in Research","doi-asserted-by":"crossref","award":["NC\/W001438\/1"],"award-info":[{"award-number":["NC\/W001438\/1"]}],"id":[{"id":"10.13039\/501100000849","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001320","name":"Wolfson Foundation","doi-asserted-by":"crossref","award":["WLR\\R1\\170024"],"award-info":[{"award-number":["WLR\\R1\\170024"]}],"id":[{"id":"10.13039\/501100001320","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Innate immune responses to inflammation and infection are complex and represent major challenges for developing much needed new treatments for chronic inflammatory diseases and drug-resistant infections. To be ultimately successful, the immune response must be balanced to allow pathogen clearance without excess tissue damage, processes controlled by pro- and anti-inflammatory signals. The roles of anti-inflammatory signalling in raising an appropriate immune response are underappreciated, representing overlooked potential drug targets. This is especially true in neutrophils, a difficult cell type to study ex vivo owing to a short lifespan, dogmatically seen as being highly pro-inflammatory. Here, we have generated and describe the first zebrafish transgenic line [TgBAC(arg2:eGFP)sh571] that labels expression of the anti-inflammatory gene arginase 2 (arg2) and show that a subpopulation of neutrophils upregulate arginase soon after immune challenge with injury and infection. At wound-healing stages, arg2:GFP is expressed in subsets of neutrophils and macrophages, potentially representing anti-inflammatory, polarised immune cell populations. Our findings identify nuanced responses to immune challenge in vivo, responses that represent new opportunities for therapeutic interventions during inflammation and infection.<\/jats:p>","DOI":"10.1242\/dmm.049966","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T12:16:13Z","timestamp":1681992973000},"source":"Crossref","is-referenced-by-count":16,"title":["An <i>arginase 2<\/i> promoter transgenic line illuminates immune cell polarisation in zebrafish"],"prefix":"10.1242","volume":"16","author":[{"given":"Ffion R.","family":"Hammond","sequence":"first","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Lewis","sequence":"additional","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00eb C.","family":"Speirs","sequence":"additional","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Sipka","sequence":"additional","affiliation":[{"name":"LPHI, University of Montpellier, CNRS 2 , 34090 Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lewis G.","family":"Williams","sequence":"additional","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Nguyen-Chi","sequence":"additional","affiliation":[{"name":"LPHI, University of Montpellier, CNRS 2 , 34090 Montpellier , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1325-0725","authenticated-orcid":false,"given":"Annemarie H.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Institute of Biology Leiden, Leiden University 3 , Einsteinweg 55, 2333 CC Leiden , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert F.","family":"Wiegertjes","sequence":"additional","affiliation":[{"name":"Aquaculture and Fisheries Group 4 , Department of Animal Sciences , , 6700 AH Wageningen , The Netherlands"},{"name":"Wageningen University & Research 4 , Department of Animal Sciences , , 6700 AH Wageningen , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1683-0749","authenticated-orcid":false,"given":"Philip M.","family":"Elks","sequence":"additional","affiliation":[{"name":"The Bateson Centre 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"},{"name":"University of Sheffield, Western Bank 1 , Department of Infection and Immunity and Cardiovascular Disease , , Sheffield S10 2TN , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,5,3]]},"reference":[{"key":"2023051112535814900_DMM049966C1","doi-asserted-by":"publisher","first-page":"e2134","DOI":"10.1371\/journal.pntd.0002134","article-title":"Arginase activity - a marker of disease status in patients with visceral leishmaniasis in ethiopia","volume":"7","author":"Abebe","year":"2013","journal-title":"PLoS Negl. 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