{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:56:58Z","timestamp":1791230218005,"version":"4.1.0"},"reference-count":53,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82103315"],"award-info":[{"award-number":["82103315"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32100476"],"award-info":[{"award-number":["32100476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82273010"],"award-info":[{"award-number":["82273010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"crossref","award":["2208085J44"],"award-info":[{"award-number":["2208085J44"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"crossref","award":["2108085MC77"],"award-info":[{"award-number":["2108085MC77"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007121","name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["BJPY2022B09"],"award-info":[{"award-number":["BJPY2022B09"]}],"id":[{"id":"10.13039\/501100007121","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007121","name":"Hefei Institutes of Physical Science, Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["YZJJ202105"],"award-info":[{"award-number":["YZJJ202105"]}],"id":[{"id":"10.13039\/501100007121","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Organoid culture systems are very powerful models that recapitulate in vivo organ development and disease pathogenesis, offering great promise in basic research, drug screening and precision medicine. However, the application of organoids derived from patients with cancer to immunotherapeutic research is a relatively untapped area. Esophageal cancer is one of the most lethal malignancies worldwide, including two major pathological subtypes: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma. ESCC shares many biological and genomic features with oral squamous cell cancers. Herein, we provide a versatile protocol for the establishment and maintenance of oral and esophageal organoid cultures derived from both murine and human samples. We describe culture conditions for organoids derived from normal tongue, esophagus and gastroesophageal junction, esophageal cancer and Barrett's esophagus. In addition, we establish an ex vivo model by co-culturing patient tumor-derived organoids and autologous CD8+ T lymphocytes to assess CD8+ T cell-mediated tumor killing. Our protocol can also be modified for organoid establishment from other squamous epithelia and carcinomas. The co-culture model can serve as a template for studies of other tumor-immune cell interactions and the efficacy of immune checkpoint blockade therapy.<\/jats:p>","DOI":"10.1242\/dmm.050319","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T08:19:22Z","timestamp":1705997962000},"source":"Crossref","is-referenced-by-count":17,"title":["Establishing mouse and human oral esophageal organoids to investigate the tumor immune response"],"prefix":"10.1242","volume":"17","author":[{"given":"Yuan","family":"Jiang","sequence":"first","affiliation":[{"name":"Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences 1 , Hefei 230031 , China"},{"name":"University of Science and Technology of China 2 , Hefei 230026 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology, Herman Ostrow 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Science and Technology, Anhui University 5 , Hefei 230601 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiu","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences 1 , Hefei 230031 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine 4 Division of Gastroenterology and Hepatology, Department of Medicine and Oncology , , Baltimore, MD 21287 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences 1 , Hefei 230031 , China"},{"name":"University of Science and Technology of China 2 , Hefei 230026 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences 1 , Hefei 230031 , China"},{"name":"University of Science and Technology of China 2 , Hefei 230026 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kalra","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine 4 Division of Gastroenterology and Hepatology, Department of Medicine and Oncology , , Baltimore, MD 21287 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Yang","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine 4 Division of Gastroenterology and Hepatology, Department 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