{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T11:35:06Z","timestamp":1775907306721,"version":"3.50.1"},"reference-count":113,"publisher":"The Company of Biologists","issue":"5","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Toh Kian Chui Foundation"},{"DOI":"10.13039\/501100011738","name":"Lee Kong Chian School of Medicine, Nanyang Technological University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011738","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000835","name":"University of Bath","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000835","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Heterotopic ossification is the inappropriate formation of bone in soft tissues of the body. It can manifest spontaneously in rare genetic conditions or as a response to injury, known as acquired heterotopic ossification. There are several experimental models for studying acquired heterotopic ossification from different sources of damage. However, their tenuous mechanistic relevance to the human condition, invasive and laborious nature and\/or lack of amenability to chemical and genetic screens, limit their utility. To address these limitations, we developed a simple zebrafish injury model that manifests heterotopic ossification with high penetrance in response to clinically emulating injuries, as observed in human myositis ossificans traumatica. Using this model, we defined the transcriptional response to trauma, identifying differentially regulated genes. Mutant analyses revealed that an increase in the activity of the potassium channel Kcnk5b potentiates injury response, whereas loss of function of the interleukin 11 receptor paralogue (Il11ra) resulted in a drastically reduced ossification response. Based on these findings, we postulate that enhanced ionic signalling, specifically through Kcnk5b, regulates the intensity of the skeletogenic injury response, which, in part, requires immune response regulated by Il11ra.<\/jats:p>","DOI":"10.1242\/dmm.050724","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T03:00:15Z","timestamp":1715569215000},"source":"Crossref","is-referenced-by-count":3,"title":["Genetic regulation of injury-induced heterotopic ossification in adult zebrafish"],"prefix":"10.1242","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2747-4500","authenticated-orcid":false,"given":"Arun-Kumar","family":"Kaliya-Perumal","sequence":"first","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University 1 , 59 Nanyang Drive 636921 , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8301-0172","authenticated-orcid":false,"given":"Cenk","family":"Celik","sequence":"additional","affiliation":[{"name":"Genetics Institute, University College London 2 Department of Genetics, Evolution and Environment , , London WC1E 6BT , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2371-1924","authenticated-orcid":false,"given":"Tom J.","family":"Carney","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University 1 , 59 Nanyang Drive 636921 , Singapore"},{"name":"Institute of Molecular and Cell Biology (IMCB) 3 , Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos 138673 , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7201-4693","authenticated-orcid":false,"given":"Matthew P.","family":"Harris","sequence":"additional","affiliation":[{"name":"Harvard Medical School 4 Department of Genetics , , Boston, MA 02115 , USA"},{"name":"Boston Children's Hospital 5 Department of Orthopedic Research , , Boston, MA 02115 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8224-9958","authenticated-orcid":false,"given":"Philip W.","family":"Ingham","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University 1 , 59 Nanyang Drive 636921 , Singapore"},{"name":"University of Bath 6 Department of Life Sciences , , Bath BA2 7AY , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"2024053109390079200_DMM050724C1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1097\/00003086-199102000-00005","article-title":"Periarticular heterotopic ossification after total hip arthroplasty. Risk factors and consequences","volume":"263","author":"Ahrengart","year":"1991","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C2","doi-asserted-by":"publisher","first-page":"eabg6497","DOI":"10.1126\/sciadv.abg6497","article-title":"Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration","volume":"7","author":"Allanki","year":"2021","journal-title":"Sci. Adv."},{"key":"2024053109390079200_DMM050724C3","doi-asserted-by":"publisher","first-page":"e53761","DOI":"10.7554\/eLife.53761","article-title":"Fibrodysplasia ossificans progressiva mutant ACVR1 signals by multiple modalities in the developing zebrafish","volume":"9","author":"Allen","year":"2020","journal-title":"eLife"},{"key":"2024053109390079200_DMM050724C4","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1007\/s12094-007-0006-7","article-title":"The role of radiotherapy for prevention of heterotopic ossification after major hip surgery","volume":"9","author":"Andreu Martinez","year":"2007","journal-title":"Clin. Transl. Oncol."},{"key":"2024053109390079200_DMM050724C5","first-page":"2","article-title":"Animal models for acquired heterotopic ossification","volume":"80","author":"Anthonissen","year":"2014","journal-title":"Acta Orthop. Belg."},{"key":"2024053109390079200_DMM050724C6","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.ajhg.2008.07.010","article-title":"Maternally inherited Birk Barel mental retardation dysmorphism syndrome caused by a mutation in the genomically imprinted potassium channel KCNK9","volume":"83","author":"Barel","year":"2008","journal-title":"Am. J. Hum. Genet."},{"key":"2024053109390079200_DMM050724C7","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.bone.2005.08.016","article-title":"Early changes in prostaglandins precede bone formation in a rabbit model of heterotopic ossification","volume":"38","author":"Bartlett","year":"2006","journal-title":"Bone"},{"key":"2024053109390079200_DMM050724C8","doi-asserted-by":"publisher","first-page":"S110-S119","DOI":"10.1097\/00003086-200210001-00013","article-title":"Muscle contusion injury and myositis ossificans traumatica","volume":"403 Suppl","author":"Beiner","year":"2002","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C9","doi-asserted-by":"publisher","first-page":"S297","DOI":"10.1016\/j.ydbio.2018.02.012","article-title":"Kir2.1 is important for efficient BMP signaling in mammalian face development","volume":"444","author":"Belus","year":"2018","journal-title":"Dev. Biol."},{"key":"2024053109390079200_DMM050724C10","doi-asserted-by":"publisher","first-page":"786752","DOI":"10.4061\/2011\/786752","article-title":"Loading and skeletal development and maintenance","volume":"2011","author":"Bergmann","year":"2010","journal-title":"J. Osteoporos."},{"key":"2024053109390079200_DMM050724C11","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1359\/jbmr.071030","article-title":"Dysregulated BMP signaling and enhanced osteogenic differentiation of connective tissue progenitor cells from patients with fibrodysplasia ossificans progressiva (FOP)","volume":"23","author":"Billings","year":"2008","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C12","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1097\/00000658-190309000-00014","article-title":"XIII. Myositis ossificans traumatica","volume":"38","author":"Binnie","year":"1903","journal-title":"Ann. Surg."},{"key":"2024053109390079200_DMM050724C13","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1002\/dvdy.10387","article-title":"Developmental morphology of the axial skeleton of the zebrafish, Danio rerio (Ostariophysi: Cyprinidae)","volume":"228","author":"Bird","year":"2003","journal-title":"Dev. Dyn."},{"key":"2024053109390079200_DMM050724C14","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1242\/dev.073197","article-title":"Bmp signaling regulates a dose-dependent transcriptional program to control facial skeletal development","volume":"139","author":"Bonilla-Claudio","year":"2012","journal-title":"Development"},{"key":"2024053109390079200_DMM050724C15","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1186\/1752-1947-7-90","article-title":"Heterotopic ossification in the reaming tract of a percutaneous antegrade femoral nail: a case report","volume":"7","author":"Botolin","year":"2013","journal-title":"J. Med. Case Rep."},{"key":"2024053109390079200_DMM050724C16","doi-asserted-by":"publisher","first-page":"1629","DOI":"10.2106\/00004623-197355080-00006","article-title":"Ectopic ossification following total hip replacement. Incidence and a method of classification","volume":"55","author":"Brooker","year":"1973","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/path.1700660103","article-title":"Regeneration of tendon","volume":"66","author":"Buck","year":"1953","journal-title":"J. Pathol. Bacteriol."},{"key":"2024053109390079200_DMM050724C18","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1111\/febs.16693","article-title":"Regenerative inflammation: When immune cells help to re-build tissues","volume":"291","author":"Caballero-Sanchez","year":"2022","journal-title":"FEBS J."},{"key":"2024053109390079200_DMM050724C19","doi-asserted-by":"publisher","first-page":"10225536231163466","DOI":"10.1177\/10225536231163466","article-title":"The development of a mouse model to investigate the formation of heterotopic ossification","volume":"31","author":"Cao","year":"2023","journal-title":"J. Orthop. Surg. (Hong Kong)"},{"key":"2024053109390079200_DMM050724C20","doi-asserted-by":"publisher","first-page":"611","DOI":"10.3390\/biomedicines8120611","article-title":"Genetic and acquired heterotopic ossification: a translational tale of mice and men","volume":"8","author":"Cappato","year":"2020","journal-title":"Biomedicines"},{"key":"2024053109390079200_DMM050724C21","doi-asserted-by":"publisher","first-page":"1253710","DOI":"10.1155\/2019\/1253710","article-title":"Zebrafish models of human skeletal disorders: embryo and adult swimming together","volume":"2019","author":"Carnovali","year":"2019","journal-title":"Biomed. Res. Int."},{"key":"2024053109390079200_DMM050724C22","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.bone.2017.05.001","article-title":"Utility of quantitative micro-computed tomographic analysis in zebrafish to define gene function during skeletogenesis","volume":"101","author":"Charles","year":"2017","journal-title":"Bone"},{"key":"2024053109390079200_DMM050724C23","first-page":"1277","article-title":"Experimental myositis ossificans in dogs","volume":"47","author":"Collins","year":"1965","journal-title":"J. Bone. Jt Bone Jt Surg. Am."},{"key":"2024053109390079200_DMM050724C24","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1097\/BOT.0000000000001158","article-title":"Has the proportion of combat-related amputations that develop heterotopic ossification increased?","volume":"32","author":"Daniels","year":"2018","journal-title":"J. Orthop. Trauma"},{"key":"2024053109390079200_DMM050724C25","doi-asserted-by":"publisher","first-page":"15106","DOI":"10.1073\/pnas.1900475116","article-title":"Deep evolutionary origin of limb and fin regeneration","volume":"116","author":"Darnet","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024053109390079200_DMM050724C26","doi-asserted-by":"publisher","first-page":"366ra163","DOI":"10.1126\/scitranslmed.aaf1090","article-title":"Two tissue-resident progenitor lineages drive distinct phenotypes of heterotopic ossification","volume":"8","author":"Dey","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"2024053109390079200_DMM050724C27","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.trsl.2017.06.004","article-title":"The traumatic bone: trauma-induced heterotopic ossification","volume":"186","author":"Dey","year":"2017","journal-title":"Transl. Res."},{"key":"2024053109390079200_DMM050724C28","doi-asserted-by":"publisher","first-page":"92","DOI":"10.23750\/abm.v90i1-S.8077","article-title":"Heterotopic ossification in primary total hip arthroplasty: which is the role of drainage?","volume":"90","author":"Di Benedetto","year":"2019","journal-title":"Acta Biomed."},{"key":"2024053109390079200_DMM050724C29","doi-asserted-by":"publisher","first-page":"101127","DOI":"10.1016\/j.bonr.2021.101127","article-title":"Developing a combat-relevant translatable large animal model of heterotopic ossification","volume":"15","author":"Epperson","year":"2021","journal-title":"Bone. Rep."},{"key":"2024053109390079200_DMM050724C30","doi-asserted-by":"publisher","first-page":"13416","DOI":"10.1073\/pnas.1309810110","article-title":"Translational profiling of cardiomyocytes identifies an early Jak1\/Stat3 injury response required for zebrafish heart regeneration","volume":"110","author":"Fang","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024053109390079200_DMM050724C31","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.2106\/JBJS.H.00792","article-title":"Heterotopic ossification in high-energy wartime extremity injuries: prevalence and risk factors","volume":"91","author":"Forsberg","year":"2009","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C32","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1302\/0301-620X.91B11.22305","article-title":"Impact of late surgical intervention on heterotopic ossification of the hip after traumatic neurological injury","volume":"91","author":"Genet","year":"2009","journal-title":"J. Bone Joint Surg. Br."},{"key":"2024053109390079200_DMM050724C33","doi-asserted-by":"publisher","first-page":"eaaq0681","DOI":"10.1126\/science.aat8434","article-title":"Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration","volume":"362","author":"Gerber","year":"2018","journal-title":"Science"},{"key":"2024053109390079200_DMM050724C34","doi-asserted-by":"publisher","first-page":"2332","DOI":"10.2106\/00004623-200312000-00010","article-title":"In vivo somatic cell gene transfer of an engineered Noggin mutein prevents BMP4-induced heterotopic ossification","volume":"85","author":"Glaser","year":"2003","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C35","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/35058574","article-title":"Potassium leak channels and the KCNK family of two-P-domain subunits","volume":"2","author":"Goldstein","year":"2001","journal-title":"Nat. Rev. Neurosci."},{"key":"2024053109390079200_DMM050724C36","doi-asserted-by":"publisher","first-page":"765","DOI":"10.2106\/00004623-194931040-00007","article-title":"The experimental production of ectopic cartilage and bone in the muscles of rabbits","volume":"31A","author":"Heinen","year":"1949","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C37","doi-asserted-by":"publisher","first-page":"15438","DOI":"10.1073\/pnas.1510540112","article-title":"Neofunction of ACVR1 in fibrodysplasia ossificans progressiva","volume":"112","author":"Hino","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024053109390079200_DMM050724C38","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.otsr.2014.10.021","article-title":"Clinically relevant heterotopic ossification after elbow fracture surgery: a risk factors study","volume":"101","author":"Hong","year":"2015","journal-title":"Orthop. Traumatol. Surg. Res."},{"key":"2024053109390079200_DMM050724C39","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s11658-021-00277-6","article-title":"Burn-induced heterotopic ossification from incidence to therapy: key signaling pathways underlying ectopic bone formation","volume":"26","author":"Hu","year":"2021","journal-title":"Cell. Mol. Biol. Lett."},{"key":"2024053109390079200_DMM050724C40","doi-asserted-by":"publisher","first-page":"3068","DOI":"10.2741\/2296","article-title":"Signaling and transcriptional regulation in osteoblast commitment and differentiation","volume":"12","author":"Huang","year":"2007","journal-title":"Front. Biosci."},{"key":"2024053109390079200_DMM050724C41","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1177\/0885066610384188","article-title":"Interleukin-6 in surgery, trauma, and critical care part II: clinical implications","volume":"26","author":"Jawa","year":"2011","journal-title":"J. Intensive Care Med."},{"key":"2024053109390079200_DMM050724C42","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.biochi.2021.10.014","article-title":"Musculoskeletal regeneration: A zebrafish perspective","volume":"196","author":"Kaliya-Perumal","year":"2022","journal-title":"Biochimie"},{"key":"2024053109390079200_DMM050724C43","doi-asserted-by":"publisher","first-page":"309287","DOI":"10.1155\/2011\/309287","article-title":"Animal models of typical heterotopic ossification","volume":"2011","author":"Kan","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"2024053109390079200_DMM050724C44","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1634\/stemcells.2008-0576","article-title":"Dysregulation of local stem\/progenitor cells as a common cellular mechanism for heterotopic ossification","volume":"27","author":"Kan","year":"2009","journal-title":"Stem Cells"},{"key":"2024053109390079200_DMM050724C45","doi-asserted-by":"publisher","first-page":"480","DOI":"10.4055\/cios.2014.6.4.480","article-title":"Myositis ossificans traumatica causing ankylosis of the elbow","volume":"6","author":"Kanthimathi","year":"2014","journal-title":"Clin. Orthop. Surg."},{"key":"2024053109390079200_DMM050724C46","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.berh.2007.11.007","article-title":"Fibrodysplasia ossificans progressiva","volume":"22","author":"Kaplan","year":"2008","journal-title":"Best Pract. Res. Clin. Rheumatol."},{"key":"2024053109390079200_DMM050724C47","doi-asserted-by":"publisher","first-page":"2084","DOI":"10.1359\/jbmr.2000.15.11.2084","article-title":"Progressive osseous heteroplasia","volume":"15","author":"Kaplan","year":"2000","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C48","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1111\/j.1749-6632.2011.06195.x","article-title":"Fibrodysplasia ossificans progressiva: a blueprint for metamorphosis","volume":"1237","author":"Kaplan","year":"2011","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2024053109390079200_DMM050724C49","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1242\/dmm.010280","article-title":"Fibrodysplasia ossificans progressiva: mechanisms and models of skeletal metamorphosis","volume":"5","author":"Kaplan","year":"2012","journal-title":"Dis. Model. Mech."},{"key":"2024053109390079200_DMM050724C50","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.bone.2017.07.024","article-title":"Heterotopic bone induction via BMP signaling: Potential therapeutic targets for fibrodysplasia ossificans progressiva","volume":"109","author":"Katagiri","year":"2018","journal-title":"Bone"},{"key":"2024053109390079200_DMM050724C51","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1007\/s00427-009-0276-x","article-title":"The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues","volume":"219","author":"Kawasaki","year":"2009","journal-title":"Dev. Genes Evol."},{"key":"2024053109390079200_DMM050724C52","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.bone.2009.10.009","article-title":"Temporal pattern of gene expression and histology of stress fracture healing","volume":"46","author":"Kidd","year":"2010","journal-title":"Bone"},{"key":"2024053109390079200_DMM050724C53","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1007\/978-1-4614-7657-3_19","article-title":"3D Slicer: a platform for subject-specific image analysis, visualization, and clinical support","volume-title":"Intraoperative Imaging and Image-Guided Therapy","author":"Kikinis","year":"2014"},{"key":"2024053109390079200_DMM050724C54","doi-asserted-by":"publisher","first-page":"1711","DOI":"10.3390\/diagnostics13101711","article-title":"Living with fibrodysplasia ossificans progressiva: radiological images of a patient with extensive heterotopic ossification","volume":"13","author":"Kotb","year":"2023","journal-title":"Diagnostics (Basel)"},{"key":"2024053109390079200_DMM050724C55","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1089\/zeb.2016.1398","article-title":"A zebrafish model of human fibrodysplasia ossificans progressiva","volume":"14","author":"LaBonty","year":"2017","journal-title":"Zebrafish"},{"key":"2024053109390079200_DMM050724C56","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1089\/zeb.2018.1611","article-title":"Injury of adult zebrafish expressing Acvr1l(Q204D) does not result in heterotopic ossification","volume":"15","author":"LaBonty","year":"2018","journal-title":"Zebrafish"},{"key":"2024053109390079200_DMM050724C57","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.ydbio.2019.08.016","article-title":"Integrated K+ channel and K+Cl- cotransporter functions are required for the coordination of size and proportion during development","volume":"456","author":"Lanni","year":"2019","journal-title":"Dev. Biol."},{"key":"2024053109390079200_DMM050724C58","doi-asserted-by":"publisher","first-page":"362","DOI":"10.24272\/j.issn.2095-8137.2021.044","article-title":"Molecular mechanisms of intermuscular bone development in fish: a review","volume":"42","author":"Li","year":"2021","journal-title":"Zool. Res."},{"key":"2024053109390079200_DMM050724C59","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2024053109390079200_DMM050724C60","doi-asserted-by":"publisher","first-page":"652","DOI":"10.2106\/JBJS.H.01177","article-title":"Identification of progenitor cells that contribute to heterotopic skeletogenesis","volume":"91","author":"Lounev","year":"2009","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C61","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"2024053109390079200_DMM050724C62","first-page":"19","article-title":"Discussion on myositis ossificans traumatica","volume":"17","author":"Makins","year":"1924","journal-title":"Proc. R. Soc. Med."},{"key":"2024053109390079200_DMM050724C63","doi-asserted-by":"publisher","first-page":"675331","DOI":"10.3389\/fgene.2021.675331","article-title":"Zebrafish Models for Human Skeletal Disorders","volume":"12","author":"Mari-Beffa","year":"2021","journal-title":"Front. Genet."},{"key":"2024053109390079200_DMM050724C64","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1097\/00005373-198808000-00012","article-title":"Heterotopic ossification around the hip with intramedullary nailing of the femur","volume":"28","author":"Marks","year":"1988","journal-title":"J. Trauma"},{"key":"2024053109390079200_DMM050724C65","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1007\/s11914-019-00541-x","article-title":"Inflammation in fibrodysplasia ossificans progressiva and other forms of heterotopic ossification","volume":"17","author":"Matsuo","year":"2019","journal-title":"Curr. Osteoporos Rep."},{"key":"2024053109390079200_DMM050724C66","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1002\/path.1711390403","article-title":"The effect of diphosphonates on heterotopic ossification in regenerating Achilles tendon of the mouse","volume":"139","author":"McClure","year":"1983","journal-title":"J. Pathol."},{"key":"2024053109390079200_DMM050724C67","doi-asserted-by":"publisher","first-page":"e10172","DOI":"10.1002\/jbm4.10172","article-title":"Heterotopic ossification: a comprehensive review","volume":"3","author":"Meyers","year":"2019","journal-title":"JBMR Plus"},{"key":"2024053109390079200_DMM050724C68","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1097\/00003086-199405000-00041","article-title":"Isolation of bone from muscles prevents the development of experimental callus-like heterotopic bone. A study of the interaction of bone and muscle in new bone formation","volume":"302","author":"Michelsson","year":"1994","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C69","doi-asserted-by":"publisher","first-page":"368","DOI":"10.4103\/ortho.IJOrtho_317_16","article-title":"Surgical approaches for total hip arthroplasty","volume":"51","author":"Moretti","year":"2017","journal-title":"Indian J. Orthop."},{"key":"2024053109390079200_DMM050724C70","doi-asserted-by":"publisher","first-page":"4698","DOI":"10.3390\/ijms20194698","article-title":"Development of teleost intermuscular bones undergoing intramembranous ossification based on histological-transcriptomic-proteomic data","volume":"20","author":"Nie","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"2024053109390079200_DMM050724C71","doi-asserted-by":"publisher","first-page":"99","DOI":"10.3389\/fnmol.2020.00099","article-title":"Ion channel contributions to morphological development: insights from the role of Kir2.1 in bone development","volume":"13","author":"Ozekin","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"2024053109390079200_DMM050724C72","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.bone.2016.07.023","article-title":"ACVR1-Fc suppresses BMP signaling and chondro-osseous differentiation in an in vitro model of Fibrodysplasia ossificans progressiva","volume":"92","author":"Pang","year":"2016","journal-title":"Bone"},{"key":"2024053109390079200_DMM050724C73","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"2024053109390079200_DMM050724C74","doi-asserted-by":"publisher","first-page":"e1004080","DOI":"10.1371\/journal.pgen.1004080","article-title":"Bioelectric signaling regulates size in zebrafish fins","volume":"10","author":"Perathoner","year":"2014","journal-title":"PLoS Genet."},{"key":"2024053109390079200_DMM050724C75","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1002\/dvdy.22183","article-title":"Survival of Hoxa13 homozygous mutants reveals a novel role in digit patterning and appendicular skeletal development","volume":"239","author":"Perez","year":"2010","journal-title":"Dev. Dyn."},{"key":"2024053109390079200_DMM050724C76","doi-asserted-by":"publisher","first-page":"1470","DOI":"10.3791\/1470","article-title":"RNA isolation from embryonic zebrafish and cDNA synthesis for gene expression analysis","volume":"30","author":"Peterson","year":"2009","journal-title":"J. Vis. Exp."},{"key":"2024053109390079200_DMM050724C77","doi-asserted-by":"publisher","first-page":"1891","DOI":"10.1002\/jbmr.4655","article-title":"Palovarotene for fibrodysplasia ossificans progressiva (FOP): results of a randomized, placebo-controlled, double-blind phase 2 trial","volume":"37","author":"Pignolo","year":"2022","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C78","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1002\/jbmr.4762","article-title":"Reduction of new heterotopic ossification (HO) in the open-label, phase 3 MOVE trial of palovarotene for fibrodysplasia ossificans progressiva (FOP)","volume":"38","author":"Pignolo","year":"2023","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C79","doi-asserted-by":"publisher","first-page":"1685","DOI":"10.1002\/jbmr.370","article-title":"Circulating osteogenic cells: implications for injury, repair, and regeneration","volume":"26","author":"Pignolo","year":"2011","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C80","first-page":"437","article-title":"Fibrodysplasia ossificans progressiva: diagnosis, management, and therapeutic horizons","volume":"10","author":"Pignolo","year":"2013","journal-title":"Pediatr Endocrinol. Rev."},{"key":"2024053109390079200_DMM050724C81","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/S0092-8674(01)00342-7","article-title":"Mutations in Kir2.1 cause the developmental and episodic electrical phenotypes of Andersen's syndrome","volume":"105","author":"Plaster","year":"2001","journal-title":"Cell"},{"key":"2024053109390079200_DMM050724C82","doi-asserted-by":"publisher","first-page":"1324","DOI":"10.3390\/cells9061324","article-title":"The survey of cells responsible for heterotopic ossification development in skeletal muscles-human and mouse models","volume":"9","author":"Pulik","year":"2020","journal-title":"Cells"},{"key":"2024053109390079200_DMM050724C83","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.2106\/JBJS.N.01056","article-title":"Heterotopic ossification: basic-science principles and clinical correlates","volume":"97","author":"Ranganathan","year":"2015","journal-title":"J. Bone Joint Surg. Am."},{"key":"2024053109390079200_DMM050724C84","doi-asserted-by":"publisher","first-page":"650874","DOI":"10.3389\/fgene.2021.650874","article-title":"Investigating transcriptional dynamics changes and time-dependent marker gene expression in the early period after skeletal muscle injury in rats","volume":"12","author":"Ren","year":"2021","journal-title":"Front. Genet."},{"key":"2024053109390079200_DMM050724C85","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1016\/S1095-6433(02)00244-1","article-title":"The integrated function of muscles and tendons during locomotion","volume":"133","author":"Roberts","year":"2002","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2024053109390079200_DMM050724C86","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1097\/00000658-191812000-00005","article-title":"A contribution to the study of myositis ossificans progressiva","volume":"68","author":"Rosenstirn","year":"1918","journal-title":"Ann. Surg."},{"key":"2024053109390079200_DMM050724C87","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.jcot.2021.03.005","article-title":"Myositis ossificans revisited - The largest reported case series","volume":"17","author":"Saad","year":"2021","journal-title":"J. Clin. Orthop. Trauma"},{"key":"2024053109390079200_DMM050724C88","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1093\/hmg\/ddu462","article-title":"The inward rectifier potassium channel Kir2.1 is required for osteoblastogenesis","volume":"24","author":"Sacco","year":"2015","journal-title":"Hum. Mol. Genet."},{"key":"2024053109390079200_DMM050724C89","first-page":"230","article-title":"Heterotopic ossification following traumatic brain injury and spinal cord injury: insight into the etiology and pathophysiology","volume":"12","author":"Sakellariou","year":"2012","journal-title":"J. Musculoskelet. Neuronal. Interact."},{"key":"2024053109390079200_DMM050724C90","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1093\/oso\/9780199638086.003.0004","article-title":"The morphology of larval and adult zebrafish","volume-title":"Zebrafish: A Practical Approach","author":"Schilling","year":"2002"},{"key":"2024053109390079200_DMM050724C91","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1097\/00003086-199810000-00005","article-title":"The Frank Stinchfield Award. Inhibition of heterotopic ossification with radiation therapy in an animal model","volume":"355","author":"Schneider","year":"1998","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C92","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/S0022-4804(61)80010-3","article-title":"Calcium deposits and new bone formation in muscle in rabbits","volume":"1","author":"Selle","year":"1961","journal-title":"J. Surg. Res."},{"key":"2024053109390079200_DMM050724C93","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1534\/genetics.120.303390","article-title":"Potassium channel-associated bioelectricity of the dermomyotome determines fin patterning in Zebrafish","volume":"215","author":"Silic","year":"2020","journal-title":"Genetics"},{"key":"2024053109390079200_DMM050724C94","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/joa.12728","article-title":"Comparative anatomy of zebrafish paired and median fin muscles: basis for functional, developmental, and macroevolutionary studies","volume":"232","author":"Siomava","year":"2018","journal-title":"J. Anat."},{"key":"2024053109390079200_DMM050724C95","doi-asserted-by":"publisher","first-page":"dev199384","DOI":"10.1242\/dev.199384","article-title":"longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth","volume":"148","author":"Stewart","year":"2021","journal-title":"Development"},{"key":"2024053109390079200_DMM050724C96","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/j.ajhg.2009.02.002","article-title":"Mutations in BMP4 are associated with subepithelial, microform, and overt cleft lip","volume":"84","author":"Suzuki","year":"2009","journal-title":"Am. J. Hum. Genet."},{"key":"2024053109390079200_DMM050724C97","doi-asserted-by":"publisher","first-page":"49011","DOI":"10.1074\/jbc.M207804200","article-title":"Interleukin-11 as a stimulatory factor for bone formation prevents bone loss with advancing age in mice","volume":"277","author":"Takeuchi","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2024053109390079200_DMM050724C98","doi-asserted-by":"publisher","first-page":"dmm037630","DOI":"10.1242\/dmm.037630","article-title":"Clinical pathologies of bone fracture modelled in zebrafish","volume":"12","author":"Tomecka","year":"2019","journal-title":"Dis. Model. Mech."},{"key":"2024053109390079200_DMM050724C99","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/s41467-017-00594-5","article-title":"interleukin-11 induces and maintains progenitors of different cell lineages during Xenopus tadpole tail regeneration","volume":"8","author":"Tsujioka","year":"2017","journal-title":"Nat. Commun."},{"key":"2024053109390079200_DMM050724C100","doi-asserted-by":"publisher","first-page":"1911","DOI":"10.3390\/cells9081911","article-title":"Zebrafish: a suitable tool for the study of cell signaling in bone","volume":"9","author":"Valenti","year":"2020","journal-title":"Cells"},{"key":"2024053109390079200_DMM050724C101","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v035.b01","article-title":"ggplot2:Elegant graphics for data analysis","volume":"35","author":"Valero-Mora","year":"2010","journal-title":"J. Stat. Softw."},{"key":"2024053109390079200_DMM050724C102","doi-asserted-by":"publisher","first-page":"612","DOI":"10.5435\/JAAOS-D-14-00269","article-title":"Myositis ossificans","volume":"23","author":"Walczak","year":"2015","journal-title":"J. Am. Acad. Orthop. Surg."},{"key":"2024053109390079200_DMM050724C103","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/00003086-198306000-00040","article-title":"Reactions of thigh tissues of sheep to blunt trauma","volume":"176","author":"Walton","year":"1983","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C104","doi-asserted-by":"publisher","first-page":"eaaz3090","DOI":"10.1126\/science.aaz3090","article-title":"Changes in regeneration-responsive enhancers shape regenerative capacities in vertebrates","volume":"369","author":"Wang","year":"2020","journal-title":"Science"},{"key":"2024053109390079200_DMM050724C105","doi-asserted-by":"publisher","first-page":"109380","DOI":"10.1016\/j.celrep.2021.109380","article-title":"Mechanical load regulates bone growth via periosteal Osteocrin","volume":"36","author":"Watanabe-Takano","year":"2021","journal-title":"Cell Rep."},{"key":"2024053109390079200_DMM050724C106","doi-asserted-by":"publisher","first-page":"3469","DOI":"10.1007\/s11999-011-1829-7","article-title":"Surgical technique: static intramedullary nailing of the femur and tibia without intraoperative fluoroscopy","volume":"469","author":"White","year":"2011","journal-title":"Clin. Orthop. Relat. Res."},{"key":"2024053109390079200_DMM050724C107","doi-asserted-by":"publisher","first-page":"e12107","DOI":"10.2196\/12107","article-title":"System setup to deliver air impact forces to a sheep limb: preparation for model development of blast-related heterotopic ossification","volume":"8","author":"Williams","year":"2019","journal-title":"JMIR Res. Protoc."},{"key":"2024053109390079200_DMM050724C108","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1002\/jbmr.1562","article-title":"Multipotent progenitors resident in the skeletal muscle interstitium exhibit robust BMP-dependent osteogenic activity and mediate heterotopic ossification","volume":"27","author":"Wosczyna","year":"2012","journal-title":"J. Bone Miner. Res."},{"key":"2024053109390079200_DMM050724C109","doi-asserted-by":"publisher","first-page":"100141","DOI":"10.1016\/j.xinn.2021.100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation (Camb)"},{"key":"2024053109390079200_DMM050724C110","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1242\/bio.012856","article-title":"Different ossification patterns of intermuscular bones in fish with different swimming modes","volume":"4","author":"Yao","year":"2015","journal-title":"Biol. Open"},{"key":"2024053109390079200_DMM050724C111","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1002\/ajmg.a.31092","article-title":"Andersen-Tawil syndrome: prospective cohort analysis and expansion of the phenotype","volume":"140","author":"Yoon","year":"2006","journal-title":"Am. J. Med. Genet. A"},{"key":"2024053109390079200_DMM050724C112","doi-asserted-by":"publisher","first-page":"812094","DOI":"10.3389\/fcell.2022.812094","article-title":"Periosteal skeletal stem cells and their response to bone injury","volume":"10","author":"Zhang","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"2024053109390079200_DMM050724C113","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/B978-0-323-96123-3.00001-4","article-title":"Basic knowledge and research methods","volume-title":"Bone Cell Biomechanics, Mechanobiology and Bone Diseases","author":"Zheng","year":"2024"}],"container-title":["Disease Models &amp; Mechanisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.050724\/3424825\/dmm050724.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.050724\/3467609\/dmm050724.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.050724\/3467609\/dmm050724.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:39:40Z","timestamp":1717133980000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dmm\/article\/17\/5\/dmm050724\/352251\/Genetic-regulation-of-injury-induced-heterotopic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,1]]},"references-count":113,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dmm.050724","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.02.09.579618","asserted-by":"object"}]},"ISSN":["1754-8403","1754-8411"],"issn-type":[{"value":"1754-8403","type":"print"},{"value":"1754-8411","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,1]]},"article-number":"dmm050724"}}