{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:33:33Z","timestamp":1790606013757,"version":"4.1.0"},"reference-count":104,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["108906\/Z\/15\/Z"],"award-info":[{"award-number":["108906\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Research Council","award":["MR\/W004763\/1"],"award-info":[{"award-number":["MR\/W004763\/1"]}]},{"name":"Medical Research Council","award":["MR\/S005544\/1"],"award-info":[{"award-number":["MR\/S005544\/1"]}]},{"name":"Medical Research Council","award":["MR\/X018733\/1"],"award-info":[{"award-number":["MR\/X018733\/1"]}]},{"DOI":"10.13039\/501100000848","name":"University of Edinburgh","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000848","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The salivary glands are often damaged during head and neck cancer radiotherapy. This results in chronic dry mouth, which adversely affects quality of life and for which there is no long-term cure. Mouse models of salivary gland injury are routinely used in regenerative research. However, there is no clear consensus on the radiation regime required to cause injury. Here, we analysed three regimes of \u03b3-irradiation of the submandibular salivary gland. Transcriptional analysis, immunofluorescence and flow cytometry was used to profile DNA damage, gland architecture and immune cell changes 3\u2005days after single doses of 10 or 15\u2005Gy or three doses of 5\u2005Gy. Irrespective of the regime, radiation induced comparable levels of DNA damage, cell cycle arrest, loss of glandular architecture, increased pro-inflammatory cytokines and a reduction in tissue-resident macrophages, relative to those observed in non-irradiated submandibular glands. Given these data, coupled with the fact that repeated anaesthetic can negatively affect animal welfare and interfere with saliva secretion, we conclude that a single dose of 10\u2005Gy irradiation is the most refined method of inducing acute salivary gland injury in a mouse model.<\/jats:p>","DOI":"10.1242\/dmm.050733","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T07:52:12Z","timestamp":1722498732000},"source":"Crossref","is-referenced-by-count":4,"title":["A single dose of radiation elicits comparable acute salivary gland injury to fractionated radiation"],"prefix":"10.1242","volume":"17","author":[{"given":"Amanda L.","family":"Johnson","sequence":"first","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia S.","family":"Elder","sequence":"additional","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"McKendrick","sequence":"additional","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"},{"name":"The Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh 2 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizi M.","family":"Hegarty","sequence":"additional","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"},{"name":"The Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh 2 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Mercer","sequence":"additional","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5902-3368","authenticated-orcid":false,"given":"Elaine","family":"Emmerson","sequence":"additional","affiliation":[{"name":"The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh 1 , 4-5 Little France Drive, Edinburgh, EH16 4UU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,8,22]]},"reference":[{"key":"2024082206580265000_DMM050733C1","doi-asserted-by":"publisher","first-page":"100401","DOI":"10.1016\/j.jbc.2021.100401","article-title":"Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double-strand break repair","volume":"296","author":"Affandi","year":"2021","journal-title":"J. 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