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We have previously shown that the course of Mycobacterium marinum infection in adult zebrafish (Danio rerio) mimics the course of tuberculosis in human. To investigate the role of the inflammasome adaptor pycard in zebrafish M. marinum infection, we produced two zebrafish knockout mutant lines for the pycard gene with CRISPR\/Cas9 mutagenesis. Although the zebrafish larvae lacking pycard developed normally and had unaltered resistance against M. marinum, the loss of pycard led to impaired survival and increased bacterial burden in the adult zebrafish. Based on histology, immune cell aggregates, granulomas, were larger in pycard-deficient fish than in wild-type controls. Transcriptome analysis with RNA sequencing of a zebrafish haematopoietic tissue, kidney, suggested a role for pycard in neutrophil-mediated defence, haematopoiesis and myelopoiesis during infection. Transcriptome analysis of fluorescently labelled, pycard-deficient kidney neutrophils identified genes that are associated with compromised resistance, supporting the importance of pycard for neutrophil-mediated immunity against M. marinum. Our results indicate that pycard is essential for resistance against mycobacteria in adult zebrafish.<\/jats:p>","DOI":"10.1242\/dmm.052061","type":"journal-article","created":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T04:10:31Z","timestamp":1738901431000},"source":"Crossref","is-referenced-by-count":6,"title":["The inflammasome adaptor\n                    <i>pycard<\/i>\n                    is essential for immunity against\n                    <i>Mycobacterium marinum<\/i>\n                    infection in adult zebrafish"],"prefix":"10.1242","volume":"18","author":[{"given":"Meri","family":"Uusi-M\u00e4kel\u00e4","sequence":"first","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanna-Kaisa Emilia","family":"Harjula","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiju","family":"Junno","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Sillanp\u00e4\u00e4","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reetta","family":"N\u00e4tkin","sequence":"additional","affiliation":[{"name":"Prostate Cancer Research Center 2 , Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]},{"name":"Tampere University 2 , Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]},{"name":"Tays Cancer Center, Tampere University Hospital 3 , FI-33521 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirja Tellervo","family":"Niskanen","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anni Karoliina","family":"Saralahti","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matti","family":"Nykter","sequence":"additional","affiliation":[{"name":"Prostate Cancer Research Center 2 , Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]},{"name":"Tampere University 2 , Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]},{"name":"Tays Cancer Center, Tampere University Hospital 3 , FI-33521 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5776-164X","authenticated-orcid":false,"given":"Mika","family":"R\u00e4met","sequence":"additional","affiliation":[{"name":"Tampere University 1 Faculty of Medicine and Health Technology , , FI-33014 Tampere ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"2025101110415281700_DMM052061C1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/emi.2016.10","article-title":"Updates on the risk factors for latent tuberculosis reactivation and their managements","volume":"5","author":"Ai","year":"2016","journal-title":"Emerg. 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