{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:11:28Z","timestamp":1790647888763,"version":"4.1.0"},"reference-count":37,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"European Research Council","award":["AdG-741888-CSI-Fun, AdG-322491-HALLMARKS-TO-THERAPY"],"award-info":[{"award-number":["AdG-741888-CSI-Fun, AdG-322491-HALLMARKS-TO-THERAPY"]}]},{"name":"European Research Council","award":["AdG-834947-CAN-IT-BARRIERS"],"award-info":[{"award-number":["AdG-834947-CAN-IT-BARRIERS"]}]},{"DOI":"10.13039\/100018694","name":"Horizon Europe Marie Sklodowska-Curie Actions","doi-asserted-by":"crossref","award":["ITN-859860-CANCERPREV"],"award-info":[{"award-number":["ITN-859860-CANCERPREV"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005788","name":"Medizinische Universit\u00e4t Wien","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005788","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ludwig Institute for Cancer Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Genetically engineered mouse models (GEMMs) are instrumental for modelling local and systemic features of complex diseases, such as cancer. Non-invasive, longitudinal cell detection and monitoring in tumors, metastases and\/or the micro-environment is paramount to achieve a better spatiotemporal understanding of cancer progression and to evaluate therapies in preclinical studies. Bioluminescent and fluorescent reporters marking tumor cells or their microenvironment are valuable for non-invasive cell detection and monitoring in vivo. Here, we report the generation of a dual reporter allele allowing simultaneous bioluminescence and fluorescence detection of cells that have undergone Cre-Lox recombination in mice. The single copy knock-in allele in the permissive collagen I locus was evaluated in the context of several cancer GEMMs, where Cre expression was achieved genetically or by ectopic virus-mediated delivery. The new reporter allele was also combined with gene-targeted alleles widely used in bone, prostate, brain and pancreas cancer research, as well as with alleles inserted into the commonly used Rosa26 and collagen I loci. This allele is, therefore, a useful addition to the portfolio of reporters to help advance preclinical research.<\/jats:p>","DOI":"10.1242\/dmm.052130","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T12:43:22Z","timestamp":1735821802000},"source":"Crossref","is-referenced-by-count":3,"title":["A new effLuc\/Kate dual reporter allele for tumor imaging in mice"],"prefix":"10.1242","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6300-2420","authenticated-orcid":false,"given":"Latifa","family":"Bakiri","sequence":"first","affiliation":[{"name":"Medical University of Vienna 1 Laboratory Genes and Disease, Department of Laboratory Medicine , , Vienna (MUW), Spitalgasse 23, 1090 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7128-4061","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Tichet","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Lausanne Branch; Swiss Institute for Experimental Cancer Research (ISREC), EPFL; Swiss Cancer Center Leman (SCCL); Agora Translational Cancer Research Center 2 , Rue du Bugnon 25A, 1005 Lausanne ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Marques","sequence":"additional","affiliation":[{"name":"Spanish National Cancer Research Centre 3 Seve Ballesteros Foundation Brain Tumor Group , , Melchor Fern\u00e1ndez Almagro, 3, 28029 Madrid ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5055-7531","authenticated-orcid":false,"given":"Martin K.","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Aarhus University 4 Department of Biomedicine , , H\u00f8egh-Guldbergs Gade 10, 8000 Aarhus ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Allen","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Lausanne Branch; Swiss Institute for Experimental Cancer Research (ISREC), EPFL; Swiss Cancer Center Leman (SCCL); Agora Translational Cancer Research Center 2 , Rue du Bugnon 25A, 1005 Lausanne ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8001-3909","authenticated-orcid":false,"given":"Stefanie","family":"Stolzlechner","sequence":"additional","affiliation":[{"name":"Center for Cancer Research, Medical University of Vienna (MUW) 5 Laboratory Bone Cancer Metastasis, Cellular and Molecular Tumor Biology , , Spitalgasse 23, 1090 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Cheng","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Lausanne Branch; Swiss Institute for Experimental Cancer Research (ISREC), EPFL; Swiss Cancer Center Leman (SCCL); Agora Translational Cancer Research Center 2 , Rue du Bugnon 25A, 1005 Lausanne ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8982-5399","authenticated-orcid":false,"given":"Kazuhiko","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Center for Cancer Research, Medical University of Vienna (MUW) 5 Laboratory Bone Cancer Metastasis, Cellular and Molecular Tumor Biology , , Spitalgasse 23, 1090 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4593-3790","authenticated-orcid":false,"given":"Massimo","family":"Squatrito","sequence":"additional","affiliation":[{"name":"Spanish National Cancer Research Centre 3 Seve Ballesteros Foundation Brain Tumor Group , , Melchor Fern\u00e1ndez Almagro, 3, 28029 Madrid ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0883-5251","authenticated-orcid":false,"given":"Douglas","family":"Hanahan","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Lausanne Branch; Swiss Institute for Experimental Cancer Research (ISREC), EPFL; Swiss Cancer Center Leman (SCCL); Agora Translational Cancer Research Center 2 , Rue du Bugnon 25A, 1005 Lausanne ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7872-0196","authenticated-orcid":false,"given":"Erwin F.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Medical University of Vienna 1 Laboratory Genes and Disease, Department of Laboratory Medicine , , Vienna (MUW), Spitalgasse 23, 1090 Vienna ,","place":["Austria"]},{"name":"Medical University of Vienna (MUW) 6 Laboratory Genes and Disease, Department of Dermatology , , Spitalgasse 23, 1090 Vienna ,","place":["Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"2025013110011748600_DMM052130C1","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1084\/jem.20160935","article-title":"Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation","volume":"214","author":"Bakiri","year":"2017","journal-title":"J. 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