{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:08:01Z","timestamp":1785510481086,"version":"3.56.0"},"reference-count":36,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NIH R01 AI155664"],"award-info":[{"award-number":["NIH R01 AI155664"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Johns Hopkins University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Mycobacterium abscessus is a fast-growing non-tuberculous mycobacterium that can cause chronic lung disease leading to rapid decline in lung function. There are no FDA-approved therapies for this disease. To support the development of new treatments, an animal model of M. abscessus lung infection that is simple to implement and requires minimal resources is crucial to encourage broad adoption. We present a mouse model using the immunocompetent BALB\/c strain, which is both widely available and cost effective. Since BALB\/c mice naturally clear M. abscessus infections, immunosuppression is necessary to sustain bacterial growth in the lungs. Once-weekly intraperitoneal injections of the immunosuppressant cyclophosphamide at 250\u2005mg\/kg successfully induced proliferation of M. abscessus during the acute phase, followed by stabilization characteristic of chronic infection. This model demonstrated the efficacy of imipenem \u2013 an antibiotic commonly used in clinical settings \u2013 by significantly reducing bacterial burdens, mirroring their effects in human cases. However, clofazimine, which is also used to treat this disease, was bacteriostatic. This cost-effective and accessible mouse model is suitable for diverse laboratory environments and provides a valuable tool for preclinical evaluation of treatments for M. abscessus lung disease.<\/jats:p>","DOI":"10.1242\/dmm.052310","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T02:40:32Z","timestamp":1745808032000},"source":"Crossref","is-referenced-by-count":5,"title":["A BALB\/c mouse model of <i>Mycobacterium abscessus<\/i> lung infection based on once-weekly cyclophosphamide administration"],"prefix":"10.1242","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3457-4523","authenticated-orcid":false,"given":"Binayak","family":"Rimal","sequence":"first","affiliation":[{"name":"Johns Hopkins University 1 Division of Infectious Diseases, Department of Medicine, School of Medicine , , Baltimore, MD 21287 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra M.","family":"Panthi","sequence":"additional","affiliation":[{"name":"Johns Hopkins University 1 Division of Infectious Diseases, Department of Medicine, School of Medicine , , Baltimore, MD 21287 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth A.","family":"Howe","sequence":"additional","affiliation":[{"name":"Johns Hopkins University 1 Division of Infectious Diseases, Department of Medicine, School of Medicine , , Baltimore, MD 21287 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-0114","authenticated-orcid":false,"given":"Gyanu","family":"Lamichhane","sequence":"additional","affiliation":[{"name":"Johns Hopkins University 1 Division of Infectious Diseases, Department of Medicine, School of Medicine , , Baltimore, MD 21287 ,","place":["USA"]},{"name":"Center for Nontuberculous Mycobacteria and Bronchiectasis, Department of Medicine, School of Medicine, Johns Hopkins University 2 , Baltimore, MD 21287 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"2025101110565791300_DMM052310C1","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1097\/QCO.0000000000000104","article-title":"Mycobacterium abscessus: challenges in diagnosis and treatment","volume":"27","author":"Benwill","year":"2014","journal-title":"Curr. 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