{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:45:14Z","timestamp":1785923114832,"version":"3.56.0"},"reference-count":82,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["390713860"],"award-info":[{"award-number":["390713860"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012957","name":"Friedrich-Schiller-Universit\u00e4t Jena","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Macrophages are essential in the defense against fungal disease. Elucidating antimicrobial mechanisms of macrophages and pathogen activities to evade these phagocytes will help in understanding fungal infections. Existing experimental models, however, have a number of disadvantages. Human macrophage-like cell lines offer genetic tractability, but often display reduced antifungal activity and altered phenotypes compared to primary cells. In contrast, primary human monocyte-derived macrophages closely reflect physiological responses but their use is constrained by donor variability, limited availability and restricted genetic manipulability. Here, we establish transdifferentiated BLaER1 cells as a human-derived, highly potent and genetically tractable infection model to study fungal\u2013host interactions. We show that BLaER1 cells display macrophage characteristics and rapidly phagocytose cells of the major fungal pathogens Candida albicans, C. glabrata and C. auris. BLaER1 cells form functional phagolysosomes and elicit pro-inflammatory immune responses. Using knockout BLaER1 cells, we demonstrate for the first time in a human macrophage model that the host factor gasdermin D and the fungal peptide toxin candidalysin have distinct roles in triggering pyroptosis and inducing lytic host cell death.<\/jats:p>","DOI":"10.1242\/dmm.052865","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:01:43Z","timestamp":1781506903000},"source":"Crossref","is-referenced-by-count":0,"title":["Transdifferentiated BLaER1 cells as a genetically tractable model to study the interaction of pathogenic fungi with macrophages"],"prefix":"10.1242","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3530-0130","authenticated-orcid":false,"given":"Johannes","family":"Sonnberger","sequence":"first","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"B\u00f6hm","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janik Adriana","family":"Tom\u00e1s-Morales","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Lange","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]},{"name":"Health and Medical University Erfurt 2 Department of Medicine , , 99089 Erfurt ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Bagola","sequence":"additional","affiliation":[{"name":"Paul Ehrlich Institute Langen 3 Division of Immunology , , 63225 Langen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Denner","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Kasper","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]},{"name":"Institute of Novel and Emerging Infectious Diseases, Friedrich Loeffler Institute, Federal Research Institute for Animal Health 4 , 17493 Greifswald-Insel Riems ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Brunke","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ger","family":"van Zandbergen","sequence":"additional","affiliation":[{"name":"Paul Ehrlich Institute Langen 3 Division of Immunology , , 63225 Langen ,","place":["Germany"]},{"name":"Institut for Immunology, University Medical Center, Johannes Gutenberg University 5 , 55131 Mainz ,","place":["Germany"]},{"name":"Research Center for Immunotherapy (FZI), University Medical Center, Johannes Gutenberg University 6 , 55131 Mainz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob L.","family":"Sprague","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]},{"name":"Institute of Experimental Biomedicine, Chair I, University Hospital W\u00fcrzburg 7 , 97080 Wu\u0308rzburg ,","place":["Germany"]},{"name":"Rudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-Universit\u00e4t 8 , 97080 W\u00fcrzburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6028-0425","authenticated-orcid":false,"given":"Bernhard","family":"Hube","sequence":"additional","affiliation":[{"name":"Hans Kn\u00f6ll Institute 1 Department of Microbial Pathogenicity Mechanisms , , 07745 Jena ,","place":["Germany"]},{"name":"Institute of Microbiology 9 , , 07743 Jena ,","place":["Germany"]},{"name":"Friedrich Schiller University 9 , , 07743 Jena ,","place":["Germany"]},{"name":"Cluster of Excellence, Balance of the Microverse, Friedrich Schiller University 10 , 07745 Jena 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