{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:47:39Z","timestamp":1792399659061,"version":"4.4.0"},"reference-count":0,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Institute of Neurological Disorders and Stroke","award":["NS111719"],"award-info":[{"award-number":["NS111719"]}]},{"name":"Hope Center for Neurological Disorders"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Peripheral nerve injury caused by cut, crush, or excessive stretch can lead to disordered regeneration and painful neuroma formation. Regrowing axons may terminate in a swollen, hypersensitive nerve stump, producing pain that can outweigh sensory or motor deficits. Despite the frequency of neuromas, treatment outcomes remain inconsistent, and the mechanisms that initiate and sustain neuroma-associated pain are poorly understood. A proximal nerve crush performed upstream of a neuroma or transection may reshape axonal growth and interrupt retrograde injury signaling, potentially promoting a regenerative, pain-resolving state. However, its long-term effects on dorsal root ganglion (DRG) programs remain unclear. Here, we use single-cell RNA sequencing to build an atlas of DRG remodeling after neuroma-forming injury and compare it with two interventions: proximal nerve crush and nerve resection. By resolving transcriptional responses across sensory neurons, satellite glial cells, Schwann cells, and macrophages, we identify intervention-specific glial, neuronal, and immune programs that distinguish regenerative from persistent injury-associated states and nominate pathways that may guide mechanism-based therapies for painful neuromas.<\/jats:p>","DOI":"10.1242\/dmm.053052","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:28:56Z","timestamp":1792398536000},"source":"Crossref","is-referenced-by-count":0,"title":["Single-cell atlas of neuroma-induced DRG remodeling reveals intervention-specific glial and immune programs"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9409-218X","authenticated-orcid":false,"given":"Casey L.","family":"Stewart","sequence":"first","affiliation":[{"name":"1 Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Morris","sequence":"additional","affiliation":[{"name":"1 Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6741-7140","authenticated-orcid":false,"given":"Alexandra E.","family":"Halevi","sequence":"additional","affiliation":[{"name":"2 Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4951-1325","authenticated-orcid":false,"given":"Amy M.","family":"Moore","sequence":"additional","affiliation":[{"name":"3 Department of Plastic and Reconstructive Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9978-050X","authenticated-orcid":false,"given":"Valeria","family":"Cavalli","sequence":"additional","affiliation":[{"name":"4 Department of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"5 Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"6 Hope Center for Neurological Disorders, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"7 Pain Center, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5826-0134","authenticated-orcid":false,"given":"Oshri","family":"Avraham","sequence":"additional","affiliation":[{"name":"1 Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,10,19]]},"container-title":["Disease Models &amp; Mechanisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.053052\/3722030\/dmm053052.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dmm\/article-pdf\/doi\/10.1242\/dmm.053052\/3722030\/dmm053052.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:28:57Z","timestamp":1792398537000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dmm\/article\/doi\/10.1242\/dmm.053052\/373210\/Single-cell-atlas-of-neuroma-induced-DRG"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,19]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1242\/dmm.053052","relation":{},"ISSN":["1754-8403","1754-8411"],"issn-type":[{"value":"1754-8403","type":"print"},{"value":"1754-8411","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,19]]},"article-number":"dmm.053052"}}