{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T04:35:35Z","timestamp":1775190935802,"version":"3.50.1"},"reference-count":31,"publisher":"The Company of Biologists","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,1]]},"abstract":"<jats:p>Dual roles of calsequestrin (CSQ-1) being the Ca2+ donor and Ca2+ acceptor make it an excellent Ca2+-buffering protein within the sarcoplasmic reticulum (SR). We have isolated and characterized a calsequestrin (csq-1)-null mutant in Caenorhabditis elegans. To our surprise, this mutant csq-1(jh109) showed no gross defects in muscle development or function but, however, is highly sensitive to perturbation of Ca2+ homeostasis. By taking advantage of the viable null mutant, we investigated the domains of CSQ-1 that are important for polymerization and cellular localization, and required for its correct buffering functions. In transgenic animals rescued with various CSQ-1 constructs, the in vivo patterns of polymerization and localization of several mutated calsequestrins were observed to correlate with the structure-function relationship. Our results suggest that polymerization of CSQ-1 is essential but not sufficient for correct cellular localization and function of CSQ-1. In addition, direct interaction between CSQ-1 and the ryanodine receptor (RyR) was found for the first time, suggesting that the cellular localization of CSQ-1 in C. elegans is indeed modulated by RyR through a physical interaction.<\/jats:p>","DOI":"10.1242\/jcs.001016","type":"journal-article","created":{"date-parts":[[2007,4,4]],"date-time":"2007-04-04T03:56:07Z","timestamp":1175658967000},"page":"1551-1558","source":"Crossref","is-referenced-by-count":19,"title":["Functional importance of polymerization and localization of calsequestrin in<i>C. elegans<\/i>"],"prefix":"10.1242","volume":"120","author":[{"given":"J. H.","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M.","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunasekaran","family":"Singaruvelu","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wonhae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gil Bu","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-Hwan","family":"Rho","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byung-Jae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Ran","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, College of Medicine, Kon-Kuk University, Chungju, 380-710, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Kagawa","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Okayama University, Okayama, 700-8530, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. H.","family":"Eom","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joohong","family":"Ahnn","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042520200691000_REF1","doi-asserted-by":"crossref","unstructured":"Ahnn, J. and Fire, A. (1994). A screen for genetic loci required for body-wall muscle development during embryogenesis in Caenorhabditis elegans. 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