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On the other hand, RhoT was predominantly expressed in heart and uterus and induced during neuronal differentiation of N1E-115 cells. Tc10 exogenously expressed in fibroblasts generated actin-filament-containing peripheral processes longer than the Cdc42-formed filopodia, whereas RhoT produced much longer and thicker processes containing actin filaments. Furthermore, both Tc10 and RhoT induced neurite outgrowth in PC12 and N1E-115 cells, but Cdc42 did not do this by itself. Tc10 and RhoT as well as Cdc42 bound to the N-terminal CRIB-motif-containing portion of N-WASP and activated N-WASP to induce Arp2\/3-complex-mediated actin polymerization. The formation of peripheral processes and neurites by Tc10 and RhoT was prevented by the coexpression of dominant-negative mutants of N-WASP. Thus, N-WASP is essential for the process formation and neurite outgrowth induced by Tc10 and RhoT. Neuronal differentiation of PC12 and N1E-115 cells induced by dibutyryl cyclic AMP and by serum starvation, respectively, was prevented by dominant-negative Cdc42,Tc10 and RhoT. Taken together, all these Rho family proteins are required for neuronal differentiation, but they exert their functions differentially in process formation and neurite extension. Consequently, N-WASP activated by these small GTPases mediates neuronal differentiation in addition to its recently identified role in glucose uptake.<\/jats:p>","DOI":"10.1242\/jcs.00208","type":"journal-article","created":{"date-parts":[[2002,11,28]],"date-time":"2002-11-28T03:03:16Z","timestamp":1038452596000},"page":"155-168","source":"Crossref","is-referenced-by-count":96,"title":["Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth"],"prefix":"10.1242","volume":"116","author":[{"given":"Tomoyuki","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, and Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba, Chiba 263-8522,Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, and Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba, Chiba 263-8522,Japan"},{"name":"Division of Biochemistry, Institute of Medical Science, University of Tokyo,Shirokanedai, Minatoku, Tokyo 108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Miki","sequence":"additional","affiliation":[{"name":"Division of Cancer Genomics, Institute of Medical Science, University of Tokyo, Shirokanedai, Minatoku, Tokyo 108-8639, Japan"},{"name":"PRESTO, Japan Science and Technology Corporation (JST), Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadaomi","family":"Takenawa","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, Institute of Medical Science, University of Tokyo,Shirokanedai, Minatoku, Tokyo 108-8639, Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST), Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, and Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba, Chiba 263-8522,Japan"},{"name":"CREST, Japan Science and Technology Corporation (JST), Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042523005155200_REF1","doi-asserted-by":"crossref","unstructured":"Aaronson, S. 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