{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:33:36Z","timestamp":1791124416136,"version":"4.1.0"},"reference-count":44,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:p>Dicentric chromosomes undergo breakage during mitosis as a result of the attachment of two centromeres on one sister chromatid to opposite spindle poles. Studies utilizing a conditional dicentric chromosome III in Saccharomyces cerevisiae have shown that dicentric chromosome repair occurs primarily by deletion of one centromere via a RAD52-dependent recombination pathway. We report that dicentric chromosome resolution requires RAD1, a gene involved in the single-strand annealing DNA repair pathway. We additionally show that single-strand annealing repair of a dicentric chromosome can occur in the absence of RAD52. RAD52-independent repair requires the adaptation-defective cdc5-ad allele of the yeast polo kinase and the DNA damage checkpoint gene RAD9. Dicentric chromosome breakage in cdc5-ad rad52mutant cells is associated with a prolonged mitotic arrest, during which nuclei undergo microtubule-dependent oscillations, accompanied by dynamic changes in nuclear morphology. We further demonstrate that the frequency of spontaneous direct repeat recombination is suppressed in yeast cells treated with benomyl, a drug that perturbs microtubules. Our findings indicate that microtubule-dependent processes facilitate recombination.<\/jats:p>","DOI":"10.1242\/jcs.00251","type":"journal-article","created":{"date-parts":[[2002,12,31]],"date-time":"2002-12-31T00:57:13Z","timestamp":1041296233000},"page":"561-569","source":"Crossref","is-referenced-by-count":28,"title":["Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1242","volume":"116","author":[{"given":"Douglas A.","family":"Thrower","sequence":"first","affiliation":[{"name":"Present address: Department of Molecular, Cellular, and Developmental Biology,University of California at Santa Barbara, Santa Barbara, CA 93106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Stemple","sequence":"additional","affiliation":[{"name":"Department of Biology, CB3280 University of North Carolina, Chapel Hill,NC 27599-3280, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Biology, CB3280 University of North Carolina, Chapel Hill,NC 27599-3280, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerry","family":"Bloom","sequence":"additional","affiliation":[{"name":"Department of Biology, CB3280 University of North Carolina, Chapel Hill,NC 27599-3280, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042517524091800_REF1","unstructured":"Aragon-Alcaide, L. and Strunnikov, A. 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