{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:59:57Z","timestamp":1791568797349,"version":"4.3.4"},"reference-count":26,"publisher":"The Company of Biologists","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,8,15]]},"abstract":"<jats:p>Prominin-1\/CD133 is a five-membrane-span glycoprotein that is thought to act as an organizer of plasma-membrane protrusions. Here, we report the molecular and cell-biological characterization of four novel prominin-1 splice variants isolated from a mouse testis cDNA library and referred to as prominin-1.s3 to prominin-1.s6. Compared with kidney-derived prominin-1.s1, the s3, s4 and s5 variants contain a distinct cytoplasmic C-terminal domain. The s4 and s5 variants bear, in addition, two and one inframe deletion(s), respectively, in the extracellular domains. The s6 variant displays a truncated C-terminal domain caused by a premature termination resulting from intron retention. Upon their ectopic expression in Chinese hamster ovary cells, the s3 and s6 variants were found to be concentrated in plasma-membrane protrusions, whereas the s4 and s5 variants did not reach the cell surface. Biochemical analyses suggest that most of the prominin-1 in the adult male reproductive system is expressed as the s6 variant. Immunohistological and electron microscopic analyses show that prominin-1 is: (1) confined to the apical surface of the epithelium all along the epididymal duct, with the exception of the initial segment; (2) concentrated in stereocilia of the epididymal duct epithelium; and (3) found on the tail of developing spermatozoa in seminiferous tubules. Our data suggest that prominin-1 is involved in the formation and\/or stabilization of epididymal stereocilia and the tail of spermatozoa, and hence might play a dual role in the biogenesis of spermatozoa.<\/jats:p>","DOI":"10.1242\/jcs.01315","type":"journal-article","created":{"date-parts":[[2004,8,17]],"date-time":"2004-08-17T18:43:23Z","timestamp":1092768203000},"page":"4301-4311","source":"Crossref","is-referenced-by-count":104,"title":["Identification of novel Prominin-1\/CD133 splice variants with alternative C-termini and their expression in epididymis and testis"],"prefix":"10.1242","volume":"117","author":[{"given":"Christine A.","family":"Fargeas","sequence":"first","affiliation":[{"name":"Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angret","family":"Joester","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Missol-Kolka","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Hellwig","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieland B.","family":"Huttner","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Corbeil","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"},{"name":"Medical Clinic and Polyclinic I, Technical University Dresden, Fetscherstrasse 74, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042612584424800_REF1","doi-asserted-by":"crossref","unstructured":"Bhatia, M. (2001). AC133 expression in human stem cells. Leukemia15, 1685-1688.","DOI":"10.1038\/sj.leu.2402255"},{"key":"2021042612584424800_REF2","doi-asserted-by":"crossref","unstructured":"Boesze-Battaglia, K., Hennessey, T. and Albert, A. D. (1989). Cholesterol heterogeneity in bovine rod outer segment disk membranes. J. Biol. Chem.264, 8151-8155.","DOI":"10.1016\/S0021-9258(18)83162-3"},{"key":"2021042612584424800_REF3","doi-asserted-by":"crossref","unstructured":"Brooks, D. E. (1983). Epididymal functions and their hormonal regulation. Aust. J. Biol. Sci.36, 205-221.","DOI":"10.1071\/BI9830205"},{"key":"2021042612584424800_REF4","doi-asserted-by":"crossref","unstructured":"Corbeil, D., Ro\u0308per, K., Weigmann, A. and Huttner, W. B. (1998). AC133 hematopoietic stem cell antigen - human homologue of mouse kidney prominin or distinct member of a novel protein family. 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