{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:20:55Z","timestamp":1790277655983,"version":"4.1.0"},"reference-count":49,"publisher":"The Company of Biologists","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:p>Claudins are cell adhesion molecules working at tight junctions (TJs) that are directly involved in compartmentalization in multicellular organisms. The cochlea includes a rather peculiar compartment filled with endolymph. This compartment is characterized by high K+ concentration (\u223c150 mM) and a positive endocochlear potential (\u223c90 mV; EP), both indispensable conditions for cochlear hair cells to transduce acoustic stimuli to electrical signals. These conditions are thought to be generated by the stria vascularis, which is adjacent to the endolymph compartment. The stria vascularis itself constitutes an isolated compartment delineated by two epithelial barriers, marginal and basal cell layers. Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11-\/-) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. Auditory brainstem response measurements revealed that Cld11-\/- mice suffered from deafness; although no obvious gross morphological malformations were detected in Cld11-\/- cochlea, freeze-fracture replica electron microscopy showed that TJs disappeared from basal cells of the stria vascularis. In good agreement with this, tracer experiments showed that the basal cell barrier was destroyed without affecting the marginal cell barrier. Importantly, in the endolymph compartment of Cld11-\/- cochlea, the K+ concentration was maintained around the normal level (\u223c150 mM), whereas the EP was suppressed down to \u223c30 mV. These findings indicated that the establishment of the stria vascularis compartment, especially the basal cell barrier, is indispensable for hearing ability through the generation\/maintenance of EP but not of a high K+ concentration in the endolymph.<\/jats:p>","DOI":"10.1242\/jcs.01393","type":"journal-article","created":{"date-parts":[[2004,9,29]],"date-time":"2004-09-29T21:48:05Z","timestamp":1096494485000},"page":"5087-5096","source":"Crossref","is-referenced-by-count":157,"title":["Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential"],"prefix":"10.1242","volume":"117","author":[{"given":"Shin-ichiro","family":"Kitajiri","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"},{"name":"Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Sakyo-ku, Kyoto 606-8501, Japan"},{"name":"Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuo","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihito","family":"Mineharu","sequence":"additional","affiliation":[{"name":"Department of Physiology II, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, 569-8686 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyuki","family":"Sonoda","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Furuse","sequence":"additional","affiliation":[{"name":"KAN Research Institute, Kyoto Research Park, Shimogyo-ku, Kyoto 606-8317, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Hata","sequence":"additional","affiliation":[{"name":"KAN Research Institute, Kyoto Research Park, Shimogyo-ku, Kyoto 606-8317, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sasaki","sequence":"additional","affiliation":[{"name":"KAN Research Institute, Kyoto Research Park, Shimogyo-ku, Kyoto 606-8317, Japan"},{"name":"Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine, Nishi-Shinbashi, Minato-ku, Tokyo 105, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Physiology II, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, 569-8686 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Physiology II, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, 569-8686 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juichi","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikio","family":"Furuse","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Tsukita","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan"},{"name":"Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042518463868300_","doi-asserted-by":"crossref","unstructured":"Anderson, J. 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