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Lentivirus-transduced mesenchymal stem cells retained their in vitro ability to differentiate into adipocytes, osteocytes and chondrocytes as well as into myocyte- and astrocyte-like cells. eGFP-mesenchymal stem cells were delivered systemically into minimally injured syngeneic mice. Tracking and tissue-specific differentiation were determined by PCR and immunohistochemistry, respectively. We found donor-derived hepatocytes, lung epithelial cells, myofibroblasts, myofibers and renal tubular cells in some of the recipient mice. Our data indicate that even in the absence of substantial injury, phenotypically defined murine mesenchymal stem cells could acquire tissue specific morphology and antigen expression and thus contribute to different tissue cell-types in vivo.<\/jats:p>","DOI":"10.1242\/jcs.01488","type":"journal-article","created":{"date-parts":[[2004,10,20]],"date-time":"2004-10-20T01:18:04Z","timestamp":1098235084000},"page":"5655-5664","source":"Crossref","is-referenced-by-count":230,"title":["In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions"],"prefix":"10.1242","volume":"117","author":[{"given":"Fernando","family":"Anjos-Afonso","sequence":"first","affiliation":[{"name":"Hematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena K.","family":"Siapati","sequence":"additional","affiliation":[{"name":"Hematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Hematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042518384515800_REF1","doi-asserted-by":"crossref","unstructured":"Alvarez-Dolado, M., Pardal, R., Garcia-Verdugo, J. 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