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A set of 44 stress- and senescence-associated genes were found to be differentially expressed in this model, among which clusterin\/apolipoprotein J (apo J) and transforming growth factor-\u03b21 (TGF-\u03b21). Transfection of apo J cDNA provided protection against premature senescence-inducing doses of UVB and other stressful agents. Neutralizing antibodies against TGF-\u03b21 or its receptor II (T\u03b2RII) sharply attenuated the senescence-associated features, suggesting a role for TGF-\u03b21 in UVB-induced premature senescence. Both the latent and active forms of TGF-\u03b21 were increased with time after the last UVB stress. Proteasome inhibition was ruled out as a potential mechanism of UVB-induced stress-induced premature senescence (SIPS). This model represents an alternative in vitro model in photoaging research for screening potential anti-photoaging compounds.<\/jats:p>","DOI":"10.1242\/jcs.01651","type":"journal-article","created":{"date-parts":[[2005,1,26]],"date-time":"2005-01-26T03:33:07Z","timestamp":1106710387000},"page":"743-758","source":"Crossref","is-referenced-by-count":233,"title":["Repeated exposure of human skin fibroblasts to UVB at subcytotoxic level triggers premature senescence through the TGF-\u03b21 signaling pathway"],"prefix":"10.1242","volume":"118","author":[{"given":"Florence","family":"Debacq-Chainiaux","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301line","family":"Borlon","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Pascal","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ve\u0301ronique","family":"Royer","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois","family":"Eliaers","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noe\u0308lle","family":"Ninane","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge\u0301raldine","family":"Carrard","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement \u2013 EA 3106 \u2013 Universite\u0301 Paris 7, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Friguet","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement \u2013 EA 3106 \u2013 Universite\u0301 Paris 7, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"de Longueville","sequence":"additional","affiliation":[{"name":"Eppendorf Array Technologies, Rue du Se\u0301minaire, 12, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Boffe","sequence":"additional","affiliation":[{"name":"Eppendorf Array Technologies, Rue du Se\u0301minaire, 12, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"Remacle","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Toussaint","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042519140835300_REF1","doi-asserted-by":"crossref","unstructured":"Angel, P., Szabowski, A. and Schorpp-Kistner, M. 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