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We employed two-colour total internal reflection microscopy to study the recruitment and dynamics of various components of the actin polymerization machinery and the epidermal growth factor receptor signalling machinery during clathrin-coated pit internalization in control cells and cells genetically deficient for functional N-WASP. We found that clathrin-coated pit endocytosis coincides with the recruitment of N-WASP, Arp2\/3 complex and associated proteins, but not of WAVE family members. Actin accumulation at clathrin-coated pits requires the Arp2\/3 complex, since Arp2\/3 complex sequestration in the cytosol abolished any detectable actin assembly. The absence of N-WASP caused a significant reduction in the frequencies of actin and Arp2\/3 complex accumulations at sites of clathrin-coated pit invagination and vesicle departure. Although N-WASP was not essential for Arp2\/3-complex-mediated actin assembly at these sites or for EGF receptor-mediated endocytosis, N-WASP deficiency caused a marked reduction of EGF internalization.<\/jats:p><jats:p>We conclude that the assembly of WASP subfamily proteins and associated factors at sites of clathrin-coated pit invagination amplifies actin accumulations at these sites promoting efficient internalization of ligands via clathrin-mediated endocytosis.<\/jats:p>","DOI":"10.1242\/jcs.02444","type":"journal-article","created":{"date-parts":[[2005,6,29]],"date-time":"2005-06-29T00:33:58Z","timestamp":1120005238000},"page":"3103-3115","source":"Crossref","is-referenced-by-count":161,"title":["N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits"],"prefix":"10.1242","volume":"118","author":[{"given":"Stefanie","family":"Benesch","sequence":"first","affiliation":[{"name":"Cytoskeleton Dynamics Group, German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Polo","sequence":"additional","affiliation":[{"name":"IFOM, the FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank P. L.","family":"Lai","sequence":"additional","affiliation":[{"name":"Cytoskeleton Dynamics Group, German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt I.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresia E. B.","family":"Stradal","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Wehland","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klemens","family":"Rottner","sequence":"additional","affiliation":[{"name":"Cytoskeleton Dynamics Group, German Research Centre for Biotechnology (GBF), Mascheroder Weg 1, 38124 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042518581348900_REF1","doi-asserted-by":"crossref","unstructured":"Aspenstrom, P. (2002). 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