{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:18:14Z","timestamp":1791281894409,"version":"4.1.0"},"reference-count":48,"publisher":"The Company of Biologists","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,8,1]]},"abstract":"<jats:p>Application of hydrodynamic mild shear stress to adherent Dictyostelium discoideum vegetative cells triggers active actin cytoskeleton remodeling resulting in net cell movement along the flow. The average cell speed is strongly stimulated by external calcium (Ca2+, K50%=22 \u03bcM), but the directionality of the movement is almost unaffected. This calcium concentration is ten times higher than the one promoting cell adhesion to glass surfaces (K50%=2 \u03bcM). Addition of the calcium chelator EGTA or the Ca2+-channel blocker gadolinium (Gd3+) transiently stops cell movement. Monitoring the evolution of cell-surface contact area with time reveals that calcium stimulates cell speed by increasing the amplitude of both protrusion and retraction events at the cell edge, but not the frequency. As a consequence, with saturating external calcium concentrations, cells are sensitive to very low shear forces (20 pN; \u03c3=0.1 Pa). Moreover, a null-mutant lacking the unique G\u03b2 subunit does not respond to external Ca2+ changes (K50%&amp;gt;1000 \u03bcM), although the directionality of the movement is comparable with that of wild-type cells. Furthermore, cells lacking the inositol 1,4,5-trisphosphate receptor (IP3-receptor) exhibit a markedly reduced Ca2+ sensitivity. Thus, calcium release from internal stores and calcium entry through the plasma membrane modulate cell speed in response to shear stress.<\/jats:p>","DOI":"10.1242\/jcs.02461","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T18:23:54Z","timestamp":1123093434000},"page":"3445-3458","source":"Crossref","is-referenced-by-count":49,"title":["Calcium mobilization stimulates<i>Dictyostelium discoideum<\/i>shear-flow-induced cell motility"],"prefix":"10.1242","volume":"118","author":[{"given":"Se\u0301bastien","family":"Fache","sequence":"first","affiliation":[{"name":"Structures et Proprie\u0301te\u0301s des Architectures Mole\u0301culaires (UMR 5919 CNRS), De\u0301partement de Recherche Fondamentale sur la Matie\u0300re Condense\u0301e, CEA-Grenoble, DRFMC\/SI3M, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u0301re\u0301mie","family":"Dalous","sequence":"additional","affiliation":[{"name":"Structures et Proprie\u0301te\u0301s des Architectures Mole\u0301culaires (UMR 5919 CNRS), De\u0301partement de Recherche Fondamentale sur la Matie\u0300re Condense\u0301e, CEA-Grenoble, DRFMC\/SI3M, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads","family":"Engelund","sequence":"additional","affiliation":[{"name":"Image Analysis & Computer Graphics, Informatics and Mathematical Modelling, Technical University of Denmark, Richard Petersens Plads, Building 321, DK-2800 Kgs. 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