{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T02:17:43Z","timestamp":1777688263081,"version":"3.51.4"},"reference-count":35,"publisher":"The Company of Biologists","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,15]]},"abstract":"<jats:p>In salivary acinar cells, the pattern of the Ca2+ signals that regulates fluid and enzyme secretion has yet to be resolved, as there are conflicting reports in the literature. We have used a two-photon technique to directly visualize the acinar cell lumen in living fragments of exocrine tissue and simultaneously recorded agonist-induced changes in intracellular Ca2+. We show near-synchronous global Ca2+ responses in submandibular acinar cells, distinct from the typical apical to basal Ca2+ wave usually seen in rodent pancreatic acinar cells. In an effort to explain the basis of these near-synchronous global Ca2+ responses we used immunocytochemical experiments to localize luminal proteins and inositol trisphosphate receptors (InsP3Rs) in tissue fragments. Zona occludens 1 (ZO-1), a tight junction protein, shows that individual submandibular acinar cells are often nearly completely encircled by a narrow luminal structure. By contrast, in pancreatic fragments, ZO-1 staining shows short luminal branches terminating abruptly at the apical pole of single acinar cells. Co-immunostaining of InsP3Rs type 2 and type 3 showed them in the same region as ZO-1 in both exocrine tissues. Functional experiments showed that the near-synchronous global Ca2+ responses were still observed in the absence of extracellular Ca2+ and also in the presence of ryanodine. We conclude that the elaborate luminal region of submandibular cells leads to a hitherto unrecognized extensive distribution of InsP3Rs in a band around the cell and that this underlies the near-synchronous global Ca2+ response to agonists. We suggest that this may be a structural adaptation in submandibular cells to support the copious amounts of fluid secreted.<\/jats:p>","DOI":"10.1242\/jcs.02533","type":"journal-article","created":{"date-parts":[[2005,8,24]],"date-time":"2005-08-24T02:33:21Z","timestamp":1124850801000},"page":"4131-4139","source":"Crossref","is-referenced-by-count":32,"title":["Ca2+ dynamics in salivary acinar cells: distinct morphology of the acinar lumen underlies near-synchronous global Ca2+ responses"],"prefix":"10.1242","volume":"118","author":[{"given":"Olga","family":"Larina","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Thorn","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042519121834400_REF1","doi-asserted-by":"crossref","unstructured":"Argent, B. 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