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Additionally, Tuba was recruited to lipid vesicles generated by overexpression of phosphatidylinositol-4-phosphate 5-kinase type I\u03b1 (PIP5K\u03b1), localizing prominently to the head of the comets and at lower levels along the actin tail. We propose that Tuba facilitates dorsal ruffling of melanoma cells through direct interaction with actin-regulatory proteins and the recruitment of signaling molecules to lipid microdomains for the coordinated assembly of a cytoskeletal network. Knockdown of Tuba by RNA interference (RNAi) attenuated PIP5K\u03b1-generated comet formation and the invasive behavior of B16 cells, implying that Tuba function is required for certain aspects of these processes. These results suggest first that Tuba-stimulated dorsal ruffling might represent a novel mechanism for the coordination of N-WASP-dependent cytoskeletal rearrangements and second that Tuba function is implicated in motility processes.<\/jats:p>","DOI":"10.1242\/jcs.03005","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T01:48:17Z","timestamp":1149644897000},"page":"2715-2726","source":"Crossref","is-referenced-by-count":75,"title":["Tuba stimulates intracellular N-WASP-dependent actin assembly"],"prefix":"10.1242","volume":"119","author":[{"given":"Eva M.","family":"Kovacs","sequence":"first","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S.","family":"Makar","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"},{"name":"Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank B.","family":"Gertler","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042519233969100_REF1","unstructured":"Balla, T. and Varnai, P. 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