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R664X-mutant protein underwent rapid degradation and caused specifically increased turnover and impaired trafficking to the plasma membrane of the wild-type protein through hetero-oligomer formation in K562 cells. Consistent with those observations, co-expression of mutant and wild-type AE1 reduced anion transport by the wild-type protein in oocytes. Transfection studies in K562 and HEK293 cells revealed that the major pathway mediating degradation of both R664X and wild-type AE1 employed endoplasmic reticulum (ER)-associated degradation through the proteasomal pathway. Proteasomal degradation of R664X protein appeared to be independent of both ubiquitylation and N-glycosylation, and aggresome formation was not observed following proteasome inhibition. These findings indicate that AE1 R664X protein, which is associated with dominant hereditary spherocytosis, has a dominant-negative effect on the expression of wild-type AE1.<\/jats:p>","DOI":"10.1242\/jcs.03101","type":"journal-article","created":{"date-parts":[[2006,8,16]],"date-time":"2006-08-16T02:48:09Z","timestamp":1155696489000},"page":"3602-3612","source":"Crossref","is-referenced-by-count":18,"title":["Ubiquitylation-independent ER-associated degradation of an AE1 mutant associated with dominant hereditary spherocytosis in cattle"],"prefix":"10.1242","volume":"119","author":[{"given":"Daisuke","family":"Ito","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Koshino","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuto","family":"Arashiki","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Adachi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizuki","family":"Tomihari","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan"},{"name":"Laboratory of Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Tamahara","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhito","family":"Kurogi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Genetics and Breeding, Department of Animal Science, Tokyo University of Agriculture, Atsugi 243-0034, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Amano","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Genetics and Breeding, Department of Animal Science, Tokyo University of Agriculture, Atsugi 243-0034, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken-ichiro","family":"Ono","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathobiology, Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutsumi","family":"Inaba","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042519334443300_REF1","doi-asserted-by":"crossref","unstructured":"Alper, S. 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