{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:26:15Z","timestamp":1791051975587,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"<jats:p>F-BAR proteins are a newly described family of proteins with unknown physiological significance. Because F-BAR proteins, including Cdc42 interacting protein-4 (CIP4), drive membrane deformation and affect endocytosis, we investigated the role of CIP4 in GLUT4 traffic by flow cytometry in GLUT4myc-expressing L6 myoblasts (L6 GLUT4myc). L6 GLUT4myc cells express CIP4a as the predominant F-BAR protein. siRNA knockdown of CIP4 increased insulin-stimulated 14C-deoxyglucose uptake by elevating cell-surface GLUT4. Enhanced surface GLUT4 was due to decreased endocytosis, which correlated with lower transferrin internalization. Immunoprecipitation of endogenous CIP4 revealed that CIP4 interacted with N-WASp and Dynamin-2 in an insulin-dependent manner. FRET confirmed the insulin-dependent, subcellular properties of these interactions. Insulin exposure stimulated specific interactions in plasma membrane and cytosolic compartments, followed by a steady-state response that underlies the coordination of proteins needed for GLUT4 traffic. Our findings reveal a physiological function for F-BAR proteins, supporting a previously unrecognized role for the F-BAR protein CIP4 in GLUT4 endocytosis, and show that interactions between CIP4 and Dynamin-2 and between CIP4 and NWASp are spatially coordinated to promote function.<\/jats:p>","DOI":"10.1242\/jcs.041343","type":"journal-article","created":{"date-parts":[[2009,6,10]],"date-time":"2009-06-10T03:43:54Z","timestamp":1244605434000},"page":"2283-2291","source":"Crossref","is-referenced-by-count":60,"title":["The F-BAR protein CIP4 promotes GLUT4 endocytosis through bidirectional interactions with N-WASp and Dynamin-2"],"prefix":"10.1242","volume":"122","author":[{"given":"Sean M.","family":"Hartig","sequence":"first","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"},{"name":"Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhei","family":"Ishikura","sequence":"additional","affiliation":[{"name":"Cell Biology Program, Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel S.","family":"Hicklen","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanming","family":"Feng","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"},{"name":"Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth G.","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Voelker","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina S.","family":"Pichot","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Grange","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Foods and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Raphael","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Rice University, Houston, TX 77005, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Klip","sequence":"additional","affiliation":[{"name":"Cell Biology Program, Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth J.","family":"Corey","sequence":"additional","affiliation":[{"name":"Division of Pediatrics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA"},{"name":"Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042520441850300_REF1","doi-asserted-by":"crossref","unstructured":"Al-Hasani, H., Hinck, C. 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