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We have analyzed the effect of robust heat shock on distribution of eIF3a\/Tif32p\/Rpg1p and showed that it results in the formation of eIF3a accumulations containing other eIF3 subunits, known yeast SG components and small but not large ribosomal subunits and eIF2\u03b1\/Sui2p. Interestingly, under these conditions, Dcp2p and Dhh1p P-body markers also colocalized with eIF3a. Microscopic analyses of the edc3\u0394lsm4\u0394C mutant demonstrated that different scaffolding proteins are required to induce SGs upon robust heat shock as opposed to glucose deprivation. Even though eIF2\u03b1 became phosphorylated under these stress conditions, the decrease in polysomes and formation of SGs occurred independently of phosphorylation of eIF2\u03b1. We conclude that under specific stress conditions, such as robust heat shock, yeast SGs do contain eIF3 and 40S ribosomes and utilize alternative routes for their assembly.<\/jats:p>","DOI":"10.1242\/jcs.045104","type":"journal-article","created":{"date-parts":[[2009,5,26]],"date-time":"2009-05-26T21:48:52Z","timestamp":1243374532000},"page":"2078-2088","source":"Crossref","is-referenced-by-count":235,"title":["Robust heat shock induces eIF2\u03b1-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast,\n                    <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1242","volume":"122","author":[{"given":"Toma\u0301s\u030c","family":"Grous\u030cl","sequence":"first","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Frydlova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavla","family":"Vas\u030cicova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Janda","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Vojtova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kater\u030cina","family":"Mali\u0301nska\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Malcova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Nova\u0301kova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Janos\u030ckova\u0301","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leos\u030c","family":"Vala\u0301s\u030cek","sequence":"additional","affiliation":[{"name":"Laboratory of Regulation of Gene Expression, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jir\u030ci\u0301","family":"Has\u030cek","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Reproduction, Institute of Microbiology of the AS CR, v.v.i., Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042523581405500_REF1","doi-asserted-by":"crossref","unstructured":"Anderson, P. and Kedersha, N. 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