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Primary N-WASP-null keratinocytes showed reduced growth compared with control cells and enhanced expression of the gene encoding the cell-cycle inhibitor p15INK4B, a TGF\u03b2 target gene. Inhibition of TGF\u03b2 signaling blocked overexpression of p15INK4B and restored proliferation of N-WASP-deficient keratinocytes in vitro. However, induction of N-WASP gene deletion in vitro did not result in obvious changes in TGF\u03b2 signaling or growth of keratinocytes, indicating that the in vivo environment is required for the phenotype development. These data identify the actin nucleation regulator N-WASP as a novel element of hair-cycle control that modulates the antiproliferative and pro-apoptotic TGF\u03b2 pathway in keratinocytes in vivo and in vitro.<\/jats:p>","DOI":"10.1242\/jcs.053835","type":"journal-article","created":{"date-parts":[[2009,12,16]],"date-time":"2009-12-16T18:22:54Z","timestamp":1260987774000},"page":"128-140","source":"Crossref","is-referenced-by-count":31,"title":["N-WASP is a novel regulator of hair-follicle cycling that controls antiproliferative TGF\u03b2 pathways"],"prefix":"10.1242","volume":"123","author":[{"given":"Tine","family":"Lefever","sequence":"first","affiliation":[{"name":"Biomedical Institute, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"BRIC, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"University of Ghent, 9052 Gent (Zwijnaarde), Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esben","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Biomedical Institute, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"BRIC, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid","family":"Basse","sequence":"additional","affiliation":[{"name":"Biomedical Institute, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"BRIC, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Paus","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Allergology and Venerology, University of Lu\u0308beck, 23538 Lu\u0308beck, Germany"},{"name":"School of Translational Medicine, University of Manchester, Manchester, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Quondamatteo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, National University of Ireland, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alanna C.","family":"Stanley","sequence":"additional","affiliation":[{"name":"Department of Anatomy, National University of Ireland, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lutz","family":"Langbein","sequence":"additional","affiliation":[{"name":"German Cancer Research Center (DKFZ), Genetics of Skin Carcinogenesis (A110), 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Biomedical Institute, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u0308rgen","family":"Wehland","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Lommel","sequence":"additional","affiliation":[{"name":"Institute for Cell Biology, University of Bonn, 53121 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cord","family":"Brakebusch","sequence":"additional","affiliation":[{"name":"Biomedical Institute, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"BRIC, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042520565904700_REF1","doi-asserted-by":"crossref","unstructured":"Bierie, B. and Moses, H. 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