{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:35:02Z","timestamp":1791408902033,"version":"4.3.3"},"reference-count":59,"publisher":"The Company of Biologists","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:p>The retrograde transport route links early endosomes and the TGN. Several endogenous and exogenous cargo proteins use this pathway, one of which is the well-explored bacterial Shiga toxin. ADP-ribosylation factors (Arfs) are ~20 kDa GTP-binding proteins that are required for protein traffic at the level of the Golgi complex and early endosomes. In this study, we expressed mutants and protein fragments that bind to Arf-GTP to show that Arf1, but not Arf6 is required for transport of Shiga toxin from early endosomes to the TGN. We depleted six Arf1-specific ARF-GTPase-activating proteins and identified AGAP2 as a crucial regulator of retrograde transport for Shiga toxin, cholera toxin and the endogenous proteins TGN46 and mannose 6-phosphate receptor. In AGAP2-depleted cells, Shiga toxin accumulates in transferrin-receptor-positive early endosomes, suggesting that AGAP2 functions in the very early steps of retrograde sorting. A number of other intracellular trafficking pathways are not affected under these conditions. These results establish that Arf1 and AGAP2 have key trafficking functions at the interface between early endosomes and the TGN.<\/jats:p>","DOI":"10.1242\/jcs.057778","type":"journal-article","created":{"date-parts":[[2010,6,16]],"date-time":"2010-06-16T02:11:03Z","timestamp":1276654263000},"page":"2381-2390","source":"Crossref","is-referenced-by-count":27,"title":["AGAP2 regulates retrograde transport between early endosomes and the TGN"],"prefix":"10.1242","volume":"123","author":[{"given":"Yoko","family":"Shiba","sequence":"first","affiliation":[{"name":"Institut Curie \u2013 Centre de Recherche, Traffic, Signaling and Delivery Laboratory, 26 rue d'Ulm, 75248 Paris Cedex 05, France"},{"name":"UMR144 CNRS, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winfried","family":"R\u00f6mer","sequence":"additional","affiliation":[{"name":"Institut Curie \u2013 Centre de Recherche, Traffic, Signaling and Delivery Laboratory, 26 rue d'Ulm, 75248 Paris Cedex 05, France"},{"name":"UMR144 CNRS, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo A.","family":"Mardones","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Program, NICHD, National Institutes of Health, Building 18T, Room 101, Bethesda, MD 20892, USA"},{"name":"Department of Physiology, Universidad Austral de Chile, Valdivia 509-9200, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia V.","family":"Burgos","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Program, NICHD, National Institutes of Health, Building 18T, Room 101, Bethesda, MD 20892, USA"},{"name":"Department of Physiology, Universidad Austral de Chile, Valdivia 509-9200, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Lamaze","sequence":"additional","affiliation":[{"name":"Institut Curie \u2013 Centre de Recherche, Traffic, Signaling and Delivery Laboratory, 26 rue d'Ulm, 75248 Paris Cedex 05, France"},{"name":"UMR144 CNRS, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludger","family":"Johannes","sequence":"additional","affiliation":[{"name":"Institut Curie \u2013 Centre de Recherche, Traffic, Signaling and Delivery Laboratory, 26 rue d'Ulm, 75248 Paris Cedex 05, France"},{"name":"UMR144 CNRS, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042521090177600_R1","doi-asserted-by":"crossref","DOI":"10.1002\/0471143030.cb1510s32","article-title":"Measuring retrograde transport to the trans-Golgi network","author":"Amessou","year":"2006","journal-title":"Curr. 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