{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:18:32Z","timestamp":1791397112215,"version":"4.3.3"},"reference-count":78,"publisher":"The Company of Biologists","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,15]]},"abstract":"<jats:p>The polarised distribution of signals downstream from receptor tyrosine kinases (RTKs) regulates fundamental cellular processes that control cell migration, growth and morphogenesis. It is poorly understood how RTKs are involved in the localised signalling and actin remodelling required for these processes. Here, we show that the Gab1 scaffold is essential for the formation of a class of polarised actin microdomain, namely dorsal ruffles, downstream from the Met, EGF and PDGF RTKs. Gab1 associates constitutively with the actin-nucleating factor N-WASP. Following RTK activation, Gab1 recruits Nck, an activator of N-WASP, into a signalling complex localised to dorsal ruffles. Formation of dorsal ruffles requires interaction between Gab1 and Nck, and also requires functional N-WASP. Epithelial cells expressing Gab1\u0394Nck (Y407F) exhibit decreased Met-dependent Rac activation, fail to induce dorsal ruffles, and have impaired cell migration and epithelial remodelling. These data show that a Gab1-Nck signalling complex interacts with several RTKs to promote polarised actin remodelling and downstream biological responses.<\/jats:p>","DOI":"10.1242\/jcs.062570","type":"journal-article","created":{"date-parts":[[2010,3,24]],"date-time":"2010-03-24T00:25:55Z","timestamp":1269390355000},"page":"1306-1319","source":"Crossref","is-referenced-by-count":44,"title":["The Gab1 scaffold regulates RTK-dependent dorsal ruffle formation through the adaptor Nck"],"prefix":"10.1242","volume":"123","author":[{"given":"Jasmine V.","family":"Abella","sequence":"first","affiliation":[{"name":"Department of Biochemistry, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Vaillancourt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie M.","family":"Frigault","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa G.","family":"Ponzo","sequence":"additional","affiliation":[{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Department of Medicine, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Zuo","sequence":"additional","affiliation":[{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena","family":"Sangwan","sequence":"additional","affiliation":[{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Larose","sequence":"additional","affiliation":[{"name":"Department of Medicine, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morag","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Rosalind and Morris Goodman Cancer Centre, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Department of Medicine, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"},{"name":"Department of Oncology, McGill University, Montr\u00e9al, Qu\u00e9bec H3A 1A1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042521265742600_R1","doi-asserted-by":"crossref","first-page":"9632","DOI":"10.1128\/MCB.25.21.9632-9645.2005","article-title":"Met\/Hepatocyte growth factor receptor ubiquitination suppresses transformation and is required for Hrs phosphorylation","volume":"25","author":"Abella","year":"2005","journal-title":"Mol. 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Cell Sci."},{"key":"2021042521265742600_R5","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/nrm1261","article-title":"Met, metastasis, motility and more","volume":"4","author":"Birchmeier","year":"2003","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042521265742600_R6","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1128\/MCB.01770-07","article-title":"Phosphorylated YDXV motifs and Nck SH2\/SH3 adaptors act cooperatively to induce actin reorganization","volume":"28","author":"Blasutig","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"2021042521265742600_R7","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/nrm1436","article-title":"Foot and mouth: podosomes, invadopodia and circular dorsal ruffles","volume":"5","author":"Buccione","year":"2004","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042521265742600_R8","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0898-6568(02)00027-X","article-title":"The Nck family of adapter proteins: regulators of actin cytoskeleton","volume":"14","author":"Buday","year":"2002","journal-title":"Cell Signal."},{"key":"2021042521265742600_R9","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/onc.2009.363","article-title":"Differential phosphorylation of the docking protein Gab1 by c-Src and the hepatocyte growth factor receptor regulates different aspects of cell functions","volume":"29","author":"Chan","year":"2009","journal-title":"Oncogene"},{"key":"2021042521265742600_R10","doi-asserted-by":"crossref","first-page":"25171","DOI":"10.1074\/jbc.273.39.25171","article-title":"Identification of Nck family genes, chromosomal localization, expression, and signaling specificity","volume":"273","author":"Chen","year":"1998","journal-title":"J. Biol. 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Chem."},{"key":"2021042521265742600_R42","doi-asserted-by":"crossref","first-page":"8513","DOI":"10.1128\/MCB.20.22.8513-8525.2000","article-title":"The tyrosine phosphatase SHP-2 is required for sustained activation of extracellular signal-regulated kinase and epithelial morphogenesis downstream from the met receptor tyrosine kinase","volume":"20","author":"Maroun","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"2021042521265742600_R43","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1091\/mbc.e02-06-0352","article-title":"Membrane targeting of Grb2-associated binder-1 (Gab1) scaffolding protein through Src myristoylation sequence substitutes for Gab1 pleckstrin homology domain and switches an epidermal growth factor response to an invasive morphogenic program","volume":"14","author":"Maroun","year":"2003","journal-title":"Mol. Biol. 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