{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:33Z","timestamp":1791338253038,"version":"4.3.1"},"reference-count":23,"publisher":"The Company of Biologists","issue":"22","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,11,15]]},"abstract":"<jats:p>The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2\/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and trafficking away from the plasma membrane. Internalisation and directed recycling of integrins are major driving forces for invasive cell motility and potentially for cancer metastasis. Here, we describe a direct requirement for WASH and Arp2\/3-mediated actin polymerisation on the endosomal membrane system for \u03b15\u03b21 integrin recycling. WASH regulates the trafficking of endosomal \u03b15\u03b21 integrin to the plasma membrane and is fundamental for integrin-driven cell morphology changes and integrin-mediated cancer cell invasion. Thus, we implicate WASH and Arp2\/3-driven actin nucleation in receptor recycling leading to invasive motility.<\/jats:p>","DOI":"10.1242\/jcs.080986","type":"journal-article","created":{"date-parts":[[2011,11,24]],"date-time":"2011-11-24T03:11:06Z","timestamp":1322104266000},"page":"3753-3759","source":"Crossref","is-referenced-by-count":138,"title":["The Arp2\/3 activator WASH regulates \u03b15\u03b21-integrin-mediated invasive migration"],"prefix":"10.1242","volume":"124","author":[{"given":"Tobias","family":"Zech","sequence":"first","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon D. J.","family":"Calaminus","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Caswell","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather J.","family":"Spence","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Carnell","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Insall","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Norman","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M.","family":"Machesky","sequence":"additional","affiliation":[{"name":"Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042621081086500_R1","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1083\/jcb.200610076","article-title":"Syndecan-4-dependent Rac1 regulation determines directional migration in response to the extracellular matrix","volume":"177","author":"Bass","year":"2007","journal-title":"J. Cell Biol."},{"key":"2021042621081086500_R2","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1016\/j.devcel.2007.08.012","article-title":"Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments","volume":"13","author":"Caswell","year":"2007","journal-title":"Dev. Cell"},{"key":"2021042621081086500_R3","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1083\/jcb.200804140","article-title":"Rab-coupling protein coordinates recycling of alpha5beta1 integrin and EGFR1 to promote cell migration in 3D microenvironments","volume":"183","author":"Caswell","year":"2008","journal-title":"J. Cell Biol."},{"key":"2021042621081086500_R4","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/nature08779","article-title":"Systems survey of endocytosis by multiparametric image analysis","volume":"464","author":"Collinet","year":"2010","journal-title":"Nature"},{"key":"2021042621081086500_R5","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1126\/science.1064829","article-title":"Taking cell-matrix adhesions to the third dimension","volume":"294","author":"Cukierman","year":"2001","journal-title":"Science"},{"key":"2021042621081086500_R6","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.devcel.2009.09.010","article-title":"The Arp2\/3 activator WASH controls the fission of endosomes through a large multiprotein complex","volume":"17","author":"Derivery","year":"2009","journal-title":"Dev. Cell"},{"key":"2021042621081086500_R7","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/cm.20437","article-title":"WASH and the Arp2\/3 complex regulate endosome shape and trafficking","volume":"67","author":"Duleh","year":"2010","journal-title":"Cytoskeleton (Hoboken)"},{"key":"2021042621081086500_R8","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.devcel.2009.09.009","article-title":"A FAM21-containing WASH complex regulates retromer-dependent sorting","volume":"17","author":"Gomez","year":"2009","journal-title":"Dev. Cell"},{"key":"2021042621081086500_R9","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1242\/jcs.071472","article-title":"The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics","volume":"123","author":"Harbour","year":"2010","journal-title":"J. Cell Sci."},{"key":"2021042621081086500_R10","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/nrm1940","article-title":"Harnessing actin dynamics for clathrin-mediated endocytosis","volume":"7","author":"Kaksonen","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042621081086500_R11","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1111\/j.1600-0854.2008.00816.x","article-title":"Endocytosis and cancer: an \u2018insider\u2019 network with dangerous liaisons","volume":"9","author":"Lanzetti","year":"2008","journal-title":"Traffic"},{"key":"2021042621081086500_R12","first-page":"217","article-title":"Identification of genes differentially expressed in breast cancer cell line SKBR3: potential identification of new prognostic biomarkers","volume":"14","author":"Leirdal","year":"2004","journal-title":"Int. J. Mol. Med."},{"key":"2021042621081086500_R13","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.cub.2009.12.035","article-title":"The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion","volume":"20","author":"Li","year":"2010","journal-title":"Curr. Biol."},{"key":"2021042621081086500_R14","doi-asserted-by":"crossref","first-page":"e237","DOI":"10.1371\/journal.pgen.0030237","article-title":"Human subtelomeric WASH genes encode a new subclass of the WASP family","volume":"3","author":"Linardopoulou","year":"2007","journal-title":"PLoS Genet."},{"key":"2021042621081086500_R15","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1083\/jcb.138.4.913","article-title":"Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization","volume":"138","author":"Machesky","year":"1997","journal-title":"J. Cell Biol."},{"key":"2021042621081086500_R16","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/nrm1315","article-title":"Endocytic recycling","volume":"5","author":"Maxfield","year":"2004","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042621081086500_R17","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.devcel.2009.01.007","article-title":"Annexin A2-dependent polymerization of actin mediates endosome biogenesis","volume":"16","author":"Morel","year":"2009","journal-title":"Dev. Cell"},{"key":"2021042621081086500_R18","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1016\/j.cell.2009.11.026","article-title":"Mutant p53 drives invasion by promoting integrin recycling","volume":"139","author":"Muller","year":"2009","journal-title":"Cell"},{"key":"2021042621081086500_R19","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nmeth.1220","article-title":"Lifeact: a versatile marker to visualize F-actin","volume":"5","author":"Riedl","year":"2008","journal-title":"Nat. Methods"},{"key":"2021042621081086500_R20","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1128\/MCB.24.4.1540-1559.2004","article-title":"Invasion of normal human fibroblasts induced by v-Fos is independent of proliferation, immortalization, and the tumor suppressors p16INK4a and p53","volume":"24","author":"Scott","year":"2004","journal-title":"Mol. Cell Biol."},{"key":"2021042621081086500_R21","doi-asserted-by":"crossref","first-page":"16107","DOI":"10.1073\/pnas.1009471107","article-title":"Endosomal-sorting complexes required for transport (ESCRT) pathway-dependent endosomal traffic regulates the localization of active Src at focal adhesions","volume":"107","author":"Tu","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042621081086500_R22","doi-asserted-by":"crossref","first-page":"2880","DOI":"10.1091\/mbc.e10-04-0372","article-title":"WASP family proteins: their evolution and its physiological implications","volume":"21","author":"Veltman","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"2021042621081086500_R23","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1083\/jcb.200609004","article-title":"alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration","volume":"177","author":"White","year":"2007","journal-title":"J. Cell Biol."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/124\/22\/3753\/1442373\/3753.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/124\/22\/3753\/1442373\/3753.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,14]],"date-time":"2024-04-14T23:53:21Z","timestamp":1713138801000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/124\/22\/3753\/64643\/The-Arp2-3-activator-WASH-regulates-5-1-integrin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,15]]},"references-count":23,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2011,11,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.080986","relation":{},"ISSN":["1477-9137","0021-9533"],"issn-type":[{"value":"1477-9137","type":"electronic"},{"value":"0021-9533","type":"print"}],"subject":[],"published":{"date-parts":[[2011,11,15]]}}}