{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:33:21Z","timestamp":1787837601574,"version":"build-2784847793"},"reference-count":37,"publisher":"The Company of Biologists","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,6,15]]},"abstract":"<jats:p>Clathrin-mediated endocytosis (CME) involves spatially and temporally restricted molecular dynamics, to which protein kinases and actin contribute. However, whether and how these two elements merge to properly execute CME remains unknown. Here, we show that neural Wiskott\u2013Aldrich syndrome protein (N-WASP) and casein kinase 2 (CK2) form a complex and localize to clathrin-coated vesicles. N-WASP binds to and is phosphorylated by CK2, thereby reducing the kinase activity of CK2. By contrast, N-WASP-promoted actin polymerization is decreased upon both phosphorylation and binding of CK2. Knockdown of CK2 and N-WASP, either alone or in combination, causes a similar inhibition in the initial rate of CME of epidermal growth factor receptor (EGFR) and its accumulation at the plasma membrane. Increased levels of EGFR at the cell surface can only be efficiently rescued by reconstituting the N-WASP\u2013CK2 complex with either wild-type or phosphorylation-mimicking N-WASP and wild-type CK2. Notably, perturbation of N-WASP\u2013CK2 complex function showed that N-WASP controls the presence of F-actin at clathrin-coated structures. In summary, the N-WASP\u2013CK2 complex integrates in a single circuit different activities contributing to CME.<\/jats:p>","DOI":"10.1242\/jcs.081182","type":"journal-article","created":{"date-parts":[[2011,5,25]],"date-time":"2011-05-25T02:05:05Z","timestamp":1306289105000},"page":"2001-2012","source":"Crossref","is-referenced-by-count":33,"title":["Interplay between N-WASP and CK2 optimizes clathrin-mediated endocytosis of EGFR"],"prefix":"10.1242","volume":"124","author":[{"given":"Magda","family":"Galovic","sequence":"first","affiliation":[{"name":"The Netherlands Cancer Institute (NKI), Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalu","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, D-60590 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana Beatriz","family":"Areces","sequence":"additional","affiliation":[{"name":"European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"van der Kammen","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute (NKI), Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Metello","family":"Innocenti","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute (NKI), Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042521284468700_R1","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1021\/pr070500s","article-title":"Mass spectrometry analysis of a protein kinase CK2beta subunit interactome isolated from mouse brain by affinity chromatography","volume":"7","author":"Arrigoni","year":"2008","journal-title":"J. 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