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This suggests a role for this protein, an abundant annexin with a still unknown intracellular function, in starvation-induced lysosomal degradation. Transient overexpression and silencing experiments showed that annexin A5 increased lysosomal protein degradation, and colocalisation experiments, based on GFP sensitivity to lysosomal acidic pH, indicated that this was mainly the result of inducing autophagosome\u2013lysosome fusion. Annexin A5 also inhibited the endocytosis of a fluid-phase marker and cholera toxin, but not receptor-mediated endocytosis. Therefore, we propose a double and opposite role of annexin A5 in regulating the endocytic and autophagic pathways and the fusion of autophagosomes with lysosomes and endosomes.<\/jats:p>","DOI":"10.1242\/jcs.086728","type":"journal-article","created":{"date-parts":[[2012,1,21]],"date-time":"2012-01-21T05:24:36Z","timestamp":1327123476000},"page":"92-107","source":"Crossref","is-referenced-by-count":60,"title":["Annexin A5 stimulates autophagy and inhibits endocytosis"],"prefix":"10.1242","volume":"125","author":[{"given":"Ghita","family":"Ghislat","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, 46012-Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Aguado","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Centro de Investigaci\u00f3n Pr\u00edncipe Felipe, 46012-Valencia, Spain"},{"name":"CIBERER, Avda. 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