{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:38:00Z","timestamp":1791092280057,"version":"4.1.0"},"reference-count":54,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>Clathrin adaptor protein complex-1 (AP-1) and its accessory proteins play a role in the sorting of integral membrane proteins at the trans-Golgi network and endosomes. Their physiological functions in complex organisms, however, are not fully understood. In this study, we found that CG8538p, an uncharacterized Drosophila protein, shares significant structural and functional characteristics with Aftiphilin, a mammalian AP-1 accessory protein. The Drosophila Aftiphilin was shown to interact directly with the ear domain of \u03b3-adaptin of Drosophila AP-1, but not with the GAE domain of Drosophila GGA. In S2 cells, Drosophila Aftiphilin and AP-1 formed a complex and colocalized at the Golgi compartment. Moreover, tissue-specific depletion of AP-1 or Aftiphilin in the developing eyes resulted in a disordered alignment of photoreceptor neurons in larval stage and roughened eyes with aberrant ommatidia in adult flies. Furthermore, AP-1-depleted photoreceptor neurons showed an intracellular accumulation of a Notch regulator, Scabrous, and downregulation of Notch by promoting its degradation in the lysosomes. These results suggest that AP-1 and Aftiphilin are cooperatively involved in the intracellular trafficking of Notch during eye development in Drosophila.<\/jats:p>","DOI":"10.1242\/jcs.090167","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T16:11:08Z","timestamp":1330963868000},"page":"634-648","source":"Crossref","is-referenced-by-count":22,"title":["AP-1 clathrin adaptor and CG8538\/Aftiphilin are involved in Notch signaling during eye development in<i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"125","author":[{"given":"Satoshi","family":"Kametaka","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai","family":"Kametaka","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Yonekura","sequence":"additional","affiliation":[{"name":"Center of Project Research Promotion, Shinshu University, Nagano 399-4598, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mineyuki","family":"Haruta","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"},{"name":"Department of Organ Regulatory Surgery, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichi","family":"Takenoshita","sequence":"additional","affiliation":[{"name":"Department of Organ Regulatory Surgery, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo, 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Waguri","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, Fukushima Medical University, 1 Hikarigaoka, Fukushima, Fukushima 960-1295, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042522064220100_R1","doi-asserted-by":"crossref","first-page":"17411","DOI":"10.1074\/jbc.M401158200","article-title":"GGA1 interacts with the adaptor protein AP-1 through a WNSF sequence in its hinge region","volume":"279","author":"Bai","year":"2004","journal-title":"J. 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