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These results suggest that a diffusive 2D search facilitates binding of the Arp2\/3 complex to actin filaments necessary for nucleation. After nucleation, the Arp2\/3 complex integrates into the actin network and undergoes retrograde flow, which results in its broad distribution throughout the lamellipodium. By contrast, the SCAR\/WAVE complex is more restricted to the cell periphery. However, with single-molecule imaging, we also observed WAVE2 molecules undergoing retrograde motion. WAVE2 and p40 have nearly identical speeds, lifetimes and sites of network incorporation. Inhibition of actin retrograde flow does not prevent WAVE2 association and disassociation with the membrane but does inhibit WAVE2 removal from the actin cortex. Our results suggest that membrane binding and diffusion expedites the recruitment of nucleation factors to a nucleation site independent of actin assembly, but after network incorporation, ongoing actin polymerization facilitates recycling of SCAR\/WAVE and Arp2\/3 complexes.<\/jats:p>","DOI":"10.1242\/jcs.091157","type":"journal-article","created":{"date-parts":[[2012,2,20]],"date-time":"2012-02-20T20:55:17Z","timestamp":1329771317000},"page":"1165-1176","source":"Crossref","is-referenced-by-count":65,"title":["Diffusion, capture and recycling of SCAR\/WAVE and Arp2\/3 complexes observed in cells by single-molecule imaging"],"prefix":"10.1242","volume":"125","author":[{"given":"Arthur","family":"Millius","sequence":"first","affiliation":[{"name":"Cardiovascular Research Institute and Department of Biochemistry, University of California, San Francisco, CA 94158, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Laboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, 6-3 Aoba, Aramaki-Aza, Aoba-ku, Sendai, Miyagi 980-8578, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orion D.","family":"Weiner","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and Department of Biochemistry, University of California, San Francisco, CA 94158, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042522294092700_R1","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1083\/jcb.142.5.1325","article-title":"SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development","volume":"142","author":"Bear","year":"1998","journal-title":"J. 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