{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:02:24Z","timestamp":1791331344323,"version":"4.3.1"},"reference-count":49,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>An understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin MUC5AC in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen Shigella dysenteriae. Here we show that S. dysenteriae-Sinduced expression of interleukin-1\u03b2 upregulates MUC2 expression and the differential expression of MUC5AC. Differential expression of MUC5AC involves crosstalk between interleukin-1\u03b2 and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. TFF3 also downregulates E-cadherin expression, causing accumulation of \u03b2-catenin in the cytosol. Phosphorylation of GSK-3\u03b2 (inactivated) by activated Akt inhibits ubiquitylation of \u03b2-catenin, leading to its nuclear translocation, which then induces the expression of MUC5AC and cyclin D1. Accumulation of cyclin D1 alters the cell cycle, promoting cell survival and proliferation. Human colon HT29MTX cells, which overexpress MUC5AC, were resistant to adherence and invasion of S. dysenteriae when compared with other mucin-secreting HT29 cell types. Thus, during infection with S. dysenteriae, crosstalk between interleukin-1\u03b2 and Akt wired by TFF3 induces expression of MUC5AC in colonic epithelial cells. Differentially expressed gastric MUC5AC aids in mucosal clearance of S. dysenteriae, inhibiting adherence and invasion of the pathogen to colonic epithelial cells, which protects the host.<\/jats:p>","DOI":"10.1242\/jcs.092148","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T16:11:08Z","timestamp":1330963868000},"page":"703-713","source":"Crossref","is-referenced-by-count":40,"title":["Differential expression of gastric MUC5AC in colonic epithelial cells: TFF3-wired IL1 \u03b2\/Akt crosstalk-induced mucosal immune response against<i>Shigella dysenteriae<\/i>infection"],"prefix":"10.1242","volume":"125","author":[{"given":"Subramaniya Bharathi","family":"Raja","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai-600025, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malliga Raman","family":"Murali","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai-600025, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halagowder","family":"Devaraj","sequence":"additional","affiliation":[{"name":"Department of Zoology, School of Life Sciences, University of Madras, Guindy Campus, Chennai-600025, Tamilnadu, x"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivasithamparam Niranjali","family":"Devaraj","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai-600025, Tamilnadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042522065452200_R1","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1101\/gad.854900","article-title":"Phosphorylation-dependent regulation of cyclin D1 nuclear export and cyclin D1-dependent cellular transformation","volume":"14","author":"Alt","year":"2000","journal-title":"Genes Dev."},{"key":"2021042522065452200_R2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0167-7012(98)00052-9","article-title":"Quantitative assay of Salmonella adherence to intestinal epithelial cells: a new method for assessing novel intervention products","volume":"33","author":"Alwan","year":"1998","journal-title":"J. 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