{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:26:44Z","timestamp":1791343604077,"version":"4.3.1"},"reference-count":72,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1992,4,1]],"date-time":"1992-04-01T00:00:00Z","timestamp":702086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We have isolated a monoclonal antibody, 5E10, that labels discrete spots in the interphase nucleus. By immunoblotting mAb SE10 recognized predominantly a 126 kDa polypeptide with an isoelectric point of 5.5. Indirect immunofluorescence showed that mAb 5E10 labeled spots in many cell lines and tissues from rat or human origin, but not in cells from mouse, chicken, African green monkey, or the lower eukaryotes Saccharomyces and Dictyostelium. In the human bladder carcinoma cell line T24 the number of nuclear spots was found to be 21 \u00b1 10 (n=132). In many cells spots were found also in the cytoplasm. In a small fraction of T24 cells the mAb revealed thread-like structures in addition to spots. Throughout mitosis the antigen was found to be clustered in the cytoplasm, not associated with metaphase chromosomes. The spherical structures that contain the antigen were tightly bound to the nuclear matrix. Immunogold labeling with mAb 5E10 showed that the antigen is localized in 0.3 \/mi diameter spherical, electron-dense structures, reminiscent of nuclear bodies. Double-labeling experiments showed that these spots do not colocalize with U1 snRNPs and centromeres. The spots did colocalize with nuclear speckles recognized by a primary biliary cirrhosis autoimmune serum, which is thought to recognize nuclear bodies. On the basis of these observations we conclude that mAb 5E10 recognizes discrete nuclear substructures, most likely nuclear bodies.<\/jats:p>","DOI":"10.1242\/jcs.101.4.773","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T15:21:44Z","timestamp":1619364104000},"page":"773-784","source":"Crossref","is-referenced-by-count":214,"title":["A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies"],"prefix":"10.1242","volume":"101","author":[{"given":"Nico","family":"Stuurman","sequence":"first","affiliation":[{"name":"University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands 1 E. C. Slater Institute for Biochemical Research ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan De","family":"Graaf","sequence":"additional","affiliation":[{"name":"State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands 2 Department of Molecular Cell Biology ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arno","family":"Floore","sequence":"additional","affiliation":[{"name":"University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands 1 E. C. Slater Institute for Biochemical Research ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Josso","sequence":"additional","affiliation":[{"name":"University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands 1 E. C. Slater Institute for Biochemical Research ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Humbel","sequence":"additional","affiliation":[{"name":"State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands 2 Department of Molecular Cell Biology ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luitzen De","family":"Jong","sequence":"additional","affiliation":[{"name":"University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands 1 E. C. Slater Institute for Biochemical Research ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roel Van","family":"Driel","sequence":"additional","affiliation":[{"name":"University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands 1 E. C. 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