{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:09:16Z","timestamp":1791320956859,"version":"4.3.1"},"reference-count":51,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Cingulin (CGN) is a Mr 140\u2005kDa protein, which is localized in the cytoplasmic region of vertebrate tight junctions (TJ), and regulates gene expression and RhoA signalling in cultured cells. To investigate the function of CGN at the organism level, we generated CGN knockout (CGN\u2212\/\u2212) mice by homologous recombination. CGN\u2212\/\u2212 mice are viable and fertile, and are born at the expected mendelian ratios. Immunohistochemistry, immunofluorescence, electron microscopy, and permeability assays of epithelial tissues of CGN\u2212\/\u2212 mice show no cingulin labelling at junctions, normal localization of TJ proteins, and normal TJ structure and barrier function. Microarray analysis of intestinal cells does not show significant changes in gene expression between CGN\u2212\/\u2212 and CGN+\/+ mice, whereas immunoblotting analysis shows a 2-fold increase in the levels of claudin-2 protein in the duodenum and the kidney of CGN\u2212\/\u2212 mice, compared to CGN+\/+ littermates. Furthermore, CGN\u2212\/\u2212 mice show an exacerbated response to the ulcerogenic action of cysteamine, whereas acute injury of the colon by dextran sodium sulphate elicits undistinguishable responses in CGN\u2212\/\u2212 and CGN+\/+ mice. We conclude that at the organism level cingulin is dispensable for the structure and barrier function of TJ, and it is embedded in signalling networks that control the expression of claudin-2, and the mucosal response to acute injury in the duodenum.<\/jats:p>","DOI":"10.1242\/jcs.101261","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T01:11:44Z","timestamp":1346721104000},"source":"Crossref","is-referenced-by-count":40,"title":["Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury"],"prefix":"10.1242","author":[{"given":"Laurent","family":"Guillemot","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Brun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignazio","family":"Castagliuolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Pizzuti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Martines","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Jond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Bongiovanni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Citi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2012,1,1]]},"reference":[{"key":"2021042521584963400_b1","doi-asserted-by":"publisher","first-page":"9003","DOI":"10.1128\/MCB.01811-05","article-title":"Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein.","volume":"26","author":"Adachi","year":"2006","journal-title":"Mol. 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