{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T12:08:43Z","timestamp":1698754123932},"reference-count":27,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1992,6,1]],"date-time":"1992-06-01T00:00:00Z","timestamp":707356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In the human gastrointestinal epithelium, in situ hybridisation demonstrates that 12 S and 16 S mitochondrial ribosomal RNAs show maximal steady-state levels on the surface epithelial cells of the normal small intestine and colon. The mitochondrial mRNAs, cytochrome b and NADH dehydrogenase (IV) have a uniform distribution throughout the crypt and surface (villus) epithelial cells of the small intestine and colon. Histochemical stains for the activity of the mitochondrial respiratory chain enzymes succinate dehydrogenase and cytochrome oxidase also show almost uniform activities throughout the crypt-surface epithelial cell axis in the small and large intestines. In sections of normal human oesophagus the levels of mitochondrial ribosomal RNAs, mitochondrial mRNAs and the activities of mitochondrial respiratory chain enzymes are maximal over the basal cells of the stratified squamous epithelium. These results show a relative increase in mitochondrial ribosomal RNA expression compared with mitochondrial mRNAs in surface cells of simple intestinal epithelia.<\/jats:p>","DOI":"10.1242\/jcs.102.2.307","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T15:22:55Z","timestamp":1619364175000},"page":"307-314","source":"Crossref","is-referenced-by-count":6,"title":["Mitochondrial gene expression in the human gastrointestinal tract"],"prefix":"10.1242","volume":"102","author":[{"given":"Andrew J. S.","family":"Macpherson","sequence":"first","affiliation":[{"name":"1 Department of Clinical Biochemistry, King\u2019s College School of Medicine, Bessemer Road, London SE5 9PJ, UK"},{"name":"2 Department of Biochemistry, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UK"},{"name":"3 Department of Gastroenterology, Dulwich Hospital, East Dulwich Grove, London SE22, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy P.","family":"Mayall","sequence":"additional","affiliation":[{"name":"1 Department of Clinical Biochemistry, King\u2019s College School of Medicine, Bessemer Road, London SE5 9PJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry A.","family":"Chester","sequence":"additional","affiliation":[{"name":"2 Department of Biochemistry, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atta","family":"Abbasi","sequence":"additional","affiliation":[{"name":"2 Department of Biochemistry, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Forgacs","sequence":"additional","affiliation":[{"name":"3 Department of Gastroenterology, Dulwich Hospital, East Dulwich Grove, London SE22, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan D. B.","family":"Malcolm","sequence":"additional","affiliation":[{"name":"2 Department of Biochemistry, Charing Cross and Westminster Medical School, Fulham Palace Road, London W6 8RF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy J.","family":"Peters","sequence":"additional","affiliation":[{"name":"1 Department of Clinical Biochemistry, King\u2019s College School of Medicine, Bessemer Road, London SE5 9PJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1992,6,1]]},"reference":[{"key":"2023100701221027200_JOCES_102_2_307C1","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/290457a0","article-title":"Sequence and organisation of the mitochondrial genome","volume":"290","author":"Anderson","year":"1981","journal-title":"Nature"},{"key":"2023100701221027200_JOCES_102_2_307C2","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1146\/annurev.cb.04.110188.001445","article-title":"Biogenesis of mitochondria","volume":"4","author":"Attardi","year":"1988","journal-title":"Annu. Rev. Cell Biol"},{"key":"2023100701221027200_JOCES_102_2_307C3","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/0014-5793(87)81480-1","article-title":"Synthesis and turnover rates of four rat liver mitochondrial RNA species","volume":"213","author":"Cantatore","year":"1987","journal-title":"FEBS Lett"},{"key":"2023100701221027200_JOCES_102_2_307C4","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1002\/path.1711620406","article-title":"ln-situ and slot hybridisation analysis of RNA in colorectal tumours and normal colon shows distinct distributions of mitochondrial sequences","volume":"162","author":"Chester","year":"1991","journal-title":"J. Pathol"},{"key":"2023100701221027200_JOCES_102_2_307C5","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1038\/286460a0","article-title":"Distinctive sequence of human mitochondrial ribosomal RNA genes","volume":"286","author":"Eperon","year":"1980","journal-title":"Nature"},{"key":"2023100701221027200_JOCES_102_2_307C6","doi-asserted-by":"publisher","first-page":"8247","DOI":"10.1021\/bi00399a034","article-title":"Initiation codons in mammalian mitochondria: differences in the genetic code in this organelle","volume":"26","author":"Fearnley","year":"1987","journal-title":"Biochemistry"},{"key":"2023100701221027200_JOCES_102_2_307C7","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/0003-2697(83)90418-9","article-title":"A technique for radiolabelling DNA restretion endonuclease fragments to high specific activity","volume":"132","author":"Feinberg","year":"1983","journal-title":"Anal. Biochem"},{"key":"2023100701221027200_JOCES_102_2_307C8","first-page":"497","article-title":"Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger RNA species are metabolically unstable","volume":"1","author":"Gelfund","year":"1981","journal-title":"Mol. Cell Biol"},{"key":"2023100701221027200_JOCES_102_2_307C9","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1083\/jcb.108.4.1187","article-title":"Intestinal epithelial differentiation: new insights from chimeric and transgenic mice","volume":"108","author":"Gordon","year":"1989","journal-title":"J. Cell Biol"},{"key":"2023100701221027200_JOCES_102_2_307C10","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1038\/348651a0","article-title":"A mutation in the tRNA(Leu)(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies","volume":"348","author":"Goto","year":"1990","journal-title":"Nature"},{"key":"2023100701221027200_JOCES_102_2_307C11","doi-asserted-by":"publisher","first-page":"503506","DOI":"10.1042\/bj2690503","article-title":"Coordinate expression of cytochrome c oxidase subunit III and Vic mRNAs in rat tissues","volume":"269","author":"Hood","year":"1990","journal-title":"Biochem. J"},{"key":"2023100701221027200_JOCES_102_2_307C12","doi-asserted-by":"publisher","first-page":"15054","DOI":"10.1016\/S0021-9258(18)33392-1","article-title":"Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor","volume":"257","author":"Kaput","year":"1982","journal-title":"J. Biol. Chem"},{"key":"2023100701221027200_JOCES_102_2_307C13","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1016\/S0016-5085(73)80004-6","article-title":"Comparative electron microscope features of normal, hyperplastic, and adenomatous human colonic epithelium","volume":"64","author":"Kaye","year":"1973","journal-title":"Gastroenterology"},{"key":"2023100701221027200_JOCES_102_2_307C14","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/0092-8674(89)90853-2","article-title":"Termination of transcription in human mitochondria: identification and purification of a DNA binding protein factor that promotes termination","volume":"58","author":"Kruse","year":"1989","journal-title":"Cell"},{"key":"2023100701221027200_JOCES_102_2_307C15","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1203\/00006450-199008000-00011","article-title":"Progressive increase in the mutated mitochondrial DNA fraction in Kearns-Sayre syndrome","volume":"28","author":"Larsson","year":"1990","journal-title":"Peadiatr. Res"},{"key":"2023100701221027200_JOCES_102_2_307C16","doi-asserted-by":"publisher","first-page":"470474","DOI":"10.1038\/290470a0","article-title":"tRNA punctuation model of RNA processing in human mitochondria","volume":"290","author":"Ojala","year":"1981","journal-title":"Nature"},{"key":"2023100701221027200_JOCES_102_2_307C17","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1038\/347444a0","article-title":"Identification of a receptor for protein import into mitochondria","volume":"347","author":"Pain","year":"1990","journal-title":"Nature"},{"key":"2023100701221027200_JOCES_102_2_307C18","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1136\/gut.7.6.644","article-title":"An electron microscopic study of normal human sigmoid colonic mucosa","volume":"7","author":"Pittman","year":"1966","journal-title":"Gut"},{"key":"2023100701221027200_JOCES_102_2_307C19","doi-asserted-by":"publisher","first-page":"011","DOI":"10.1016\/S0021-9258(18)92920-0","article-title":"Distinctive pattern and translational control of mitochondrial protein synthesis in rat brain synaptic endings","volume":"266","author":"Polosa","year":"1991","journal-title":"J. Biol. Chem"},{"key":"2023100701221027200_JOCES_102_2_307C20","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1111\/j.1471-4159.1989.tb02518.x","article-title":"Content of mitochondrial DNA and of three mitochondrial RNAs in developing and adult rat cerebellum","volume":"58","author":"Renis","year":"1989","journal-title":"J. Neurochem"},{"key":"2023100701221027200_JOCES_102_2_307C21","volume-title":"Histology: A Text and Atlas","author":"Rbodin","year":"1974"},{"key":"2023100701221027200_JOCES_102_2_307C22","volume-title":"Molecular Cloning","author":"Sambrook","year":"1989"},{"key":"2023100701221027200_JOCES_102_2_307C23","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1002\/mds.870050406","article-title":"Mitochondrial DNA analysis in Parkinson\u2019s disease","volume":"5","author":"Shapira","year":"1990","journal-title":"Mov. Disord"},{"key":"2023100701221027200_JOCES_102_2_307C24","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1016\/0006-291X(91)91535-K","article-title":"Mitochondrial DNA mutation in a Chinese family with myoclonic epilepsy and ragged-red fibre disease","volume":"174","author":"Shih","year":"1991","journal-title":"Biochim. Biophys. Res. Commun"},{"key":"2023100701221027200_JOCES_102_2_307C25","first-page":"931","article-title":"Myoclonic epilepsy and ragged-red fibre disease (MERRF) is associated with a mitochondrial DNA tRNA(Lys) mutation","volume":"61","author":"ShofTer","year":"1990","journal-title":"Ceti"},{"key":"2023100701221027200_JOCES_102_2_307C26","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/S0074-7696(08)61401-1","article-title":"Cytology of intestinal epithelial cells","volume":"24","author":"Toner","year":"1968","journal-title":"Int. Rev. Cytol"},{"key":"2023100701221027200_JOCES_102_2_307C27","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1002\/j.1460-2075.1982.tb01209.x","article-title":"The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA","volume":"1","author":"Viebrock","year":"1982","journal-title":"EMBO J"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/102\/2\/307\/3205284\/joces_102_2_307.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/102\/2\/307\/3205284\/joces_102_2_307.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T01:22:53Z","timestamp":1696641773000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/102\/2\/307\/23296\/Mitochondrial-gene-expression-in-the-human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,1]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,6,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.102.2.307","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,1]]}}}