{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:54:41Z","timestamp":1791291281445,"version":"4.3.0"},"reference-count":41,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1993,4,1]],"date-time":"1993-04-01T00:00:00Z","timestamp":733622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We used a panel of monoclonal antibodies and immuno-cytochemistry to identify macrophages and dendritic cells in mice that are deficient in macrophage colony stimulating factor (M-CSF or CSF-1) because of the recessive osteopetrotic (op\/op) mutation. Prior work had shown that osteopetrosis is associated with a lack of osteoclasts, phagocytic cells required for remodelling in bone. Additional macrophage populations proved to be very M-CSF dependent. op\/op mice had few and some-times no peritoneal cavity phagocytes, splenic marginal zone metallophils, and lymph node subcapsular sinus macrophages. Other populations, however, reached sub-stantial levels in the absence of M-CSF, including phago-cytes in the thymic cortex, splenic red pulp, lymph node medulla, intestinal lamina propria, liver (Kupffer cells), lung (alveolar macrophages) and brain (microglia). Dendritic cells, which are specialized accessory cells for T-dependent immune responses and tolerance, were read-ily identified in skin and in the T-dependent regions of spleen, lymph node and Peyer\u2019s patch. The identifica-tion of dendritic cells utilized antibodies to MHC class II products and four different antigens that are pri-marily expressed by these accessory cells. Our findings indicate that only a few macrophage populations are critically dependent upon M-CSF in vivo. With respect to dendritic cells, the data are consistent with prior in vitro work where it was noted that GM-CSF but not M-CSF supported dendritic cell viability, function and growth.<\/jats:p>","DOI":"10.1242\/jcs.104.4.1021","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T15:37:01Z","timestamp":1619365021000},"page":"1021-1029","source":"Crossref","is-referenced-by-count":209,"title":["Identification of macrophages and dendritic cells in the osteopetrotic (op\/op) mouse"],"prefix":"10.1242","volume":"104","author":[{"given":"Margit D.","family":"Witmer-Pack","sequence":"first","affiliation":[{"name":"The Rockefeller University, New York 1 Laboratory of Cellular Physiology and Immunology , , NY 10021, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derralynn A.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology 2 , South Parks Road, Oxford OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerold","family":"Schuler","sequence":"additional","affiliation":[{"name":"University of Innsbruck 3 Dermatology , , Innsbruck A6020, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Lawson","sequence":"additional","affiliation":[{"name":"4 Department of Pharmacology, Mansfield Road, Oxford OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McWilliam","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology 2 , South Parks Road, Oxford OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayo","family":"Inaba","sequence":"additional","affiliation":[{"name":"Kyoto University 5 Department of Zoology , , Kyoto 696, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph M.","family":"Steinman","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York 1 Laboratory of Cellular Physiology and Immunology , , NY 10021, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siamon","family":"Gordon","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology 2 , South Parks Road, Oxford OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1993,4,1]]},"reference":[{"key":"2023122321442473100_JOCES_104_4_1021C1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1002\/jlb.52.1.34","article-title":"Two populations of splenic dendritic dells detected with M342, a new monoclonal to an intracellular antigen of interdigitating dendritic cells and some B lymphocytes","volume":"52","author":"Agger","year":"1992","journal-title":"J. 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