{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:27:08Z","timestamp":1789986428005,"version":"4.0.1"},"reference-count":42,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In the present study, we have examined the distribution of both hyaluronan and its receptor, CD44, during the process of endochondral ossification in the mouse tibia. Histochemical staining revealed that a large amount of hyaluronan was present in the lacunae located in the zone of hypertrophy, but it was greatly reduced or absent from the zone of erosion. In addition, hyaluronan was present in the cytoplasm of osteoprogenitor cells located in the zone of erosion. These cells also expressed CD44 on their surfaces, as revealed by double-label immunohistochem-istry. These results suggested that the osteoprogenitor cells may use CD44 to bind and internalize hyaluronan, and subsequently degrade it with lysosomal enzymes. To test this possibility, we examined the human cell line, MG-63, which closely resembles osteoprogenitor cells. These cells produced several different forms of CD44, as determined by western blotting (85, 116 and 150 kDa). In addition, the binding of isotopically labeled hyaluronan to detergent extracts of these cells was blocked by a monoclonal antibody to CD44. Similarly, the degradation of hyaluronan by these cultured cells was also inhibited by a mono-clonal antibody to CD44. To determine if these cells could remove hyaluronan from the growth plate, the cells were cultured directly on top of thin sections of the epiphysial region of long bone. After 16 hours, the sections were stained for hyaluronan. The MG-63 cells removed signifi-cant amounts of hyaluronan present in the zone of hyper-trophy, and this effect was blocked by an excess of soluble hyaluronan and by a monoclonal antibody to CD44. Taken together, these results suggest that, under physiological conditions, osteoprogenitor cells in the zone of erosion can use a CD44-dependent mechanism to remove hyaluronan.<\/jats:p>","DOI":"10.1242\/jcs.107.10.2669","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T15:49:48Z","timestamp":1619365788000},"page":"2669-2677","source":"Crossref","is-referenced-by-count":43,"title":["Distribution of hyaluronan in the epiphysial growth plate: turnover by CD44-expressing osteoprogenitor cells"],"prefix":"10.1242","volume":"107","author":[{"given":"Prasit","family":"Pavasant","sequence":"first","affiliation":[{"name":"Georgetown University Medical Center, 3900 Reservoir Road Department of Cell Biology , , Washington DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony M.","family":"Shizari","sequence":"additional","affiliation":[{"name":"Georgetown University Medical Center, 3900 Reservoir Road Department of Cell Biology , , Washington DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles B.","family":"Underhill","sequence":"additional","affiliation":[{"name":"Georgetown University Medical Center, 3900 Reservoir Road Department of Cell Biology , , Washington DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1994,10,1]]},"reference":[{"key":"2024022708241815600_JOCES_107_10_2669C1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1007\/BF00301630","article-title":"The extracellular matrix of cartilage in the growth plate before and during calcification: changes in composition and degradation of type II collagen","volume":"50","author":"Alini","year":"1992","journal-title":"Calcif. Tiss. Int."},{"key":"2024022708241815600_JOCES_107_10_2669C2","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1002\/jcp.1041530205","article-title":"Growth on type I collagen promotes expression of the osteoblastic phenotypes in human osteosarcoma MG-63 cells","volume":"153","author":"Andrianarivo","year":"1992","journal-title":"J. Cell. Physiol."},{"key":"2024022708241815600_JOCES_107_10_2669C3","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1006\/dbio.1993.1200","article-title":"TGF-\u03b21 prevent hypertrophy of epiphysial chondrocytes: regulation of gene expression for cartilage matrix proteins and metalloproteases","volume":"158","author":"Ballock","year":"1993","journal-title":"Dev. 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Int."},{"key":"2024022708241815600_JOCES_107_10_2669C7","doi-asserted-by":"publisher","first-page":"2765","DOI":"10.1083\/jcb.111.6.2765","article-title":"The hyaluronan receptor is a member of the CD44 (H- CAM) family of cell surface glycoproteins","volume":"111","author":"Culty","year":"1990","journal-title":"J. Cell Biol"},{"key":"2024022708241815600_JOCES_107_10_2669C8","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1083\/jcb.116.4.1055","article-title":"The hyaluronan receptor (CD44) participates in the uptake and degradation of hyaluronan","volume":"116","author":"Culty","year":"1992","journal-title":"J. Cell Biol."},{"key":"2024022708241815600_JOCES_107_10_2669C9","doi-asserted-by":"crossref","DOI":"10.1002\/jcp.1041600209","article-title":"Binding and degradation of hyaluronan by human breast cancer cell lines expressing different forms of CD44: Correlation with invasive potential","volume-title":"J. Cell. Physiol. (in press)","author":"Culty","year":"1994"},{"key":"2024022708241815600_JOCES_107_10_2669C10","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/S0934-8832(89)80041-1","article-title":"Association of collagenase and tissue inhibitor of metalloproteinases (TIMP) with hypertrophic cell enlargement in the growth plate","volume":"9","author":"Dean","year":"1989","journal-title":"Matrix"},{"key":"2024022708241815600_JOCES_107_10_2669C11","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1002\/jcp.1041230316","article-title":"1\u03b1,25- dihydroxyvitamin D3specific regulation of growth, morphology, and fibronectin in a human osteosarcoma cell line","volume":"123","author":"Franceschi","year":"1985","journal-title":"J. Cell. Physiol."},{"key":"2024022708241815600_JOCES_107_10_2669C12","doi-asserted-by":"publisher","first-page":"18938","DOI":"10.1016\/S0021-9258(18)37372-1","article-title":"Regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D3in human osteosarcoma cells","volume":"263","author":"Franceschi","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2024022708241815600_JOCES_107_10_2669C13","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1083\/jcb.99.6.2114","article-title":"Pericellular coat of chick embryo chondrocytes: Structural role of hyaluronate","volume":"99","author":"Goldberg","year":"1984","journal-title":"J. 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Cell Sci."},{"key":"2024022708241815600_JOCES_107_10_2669C17","first-page":"246","volume":"35","author":"Heremans","year":"1978","journal-title":"In vitro cultivation of human tumor tissues. II. Morphology and virological characterization of three cell lines Oncology"},{"key":"2024022708241815600_JOCES_107_10_2669C18","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1242\/jcs.106.1.365","article-title":"Internalization of hyaluronan by chondrocytes occurs via receptor-mediated endocytosis","volume":"106","author":"Hua","year":"1993","journal-title":"J. Cell Sci."},{"key":"2024022708241815600_JOCES_107_10_2669C19","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1002\/jbmr.5650090106","article-title":"CD44 expression in human bone: A novel marker of osteocytic differentiation","volume":"9","author":"Hughes","year":"1994","journal-title":"J. Bone Miner. Res."},{"key":"2024022708241815600_JOCES_107_10_2669C20","first-page":"125","article-title":"Structure-function relationships in bone: an examination of events at the cellular level","volume-title":"Bone and Mineral Research, annual 2","author":"Kahn","year":"1983"},{"key":"2024022708241815600_JOCES_107_10_2669C21","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1083\/jcb.120.3.825","article-title":"Hyaluronan receptor-directed assembly of chondrocyte pericellular matrix","volume":"120","author":"Knudson","year":"1993","journal-title":"J. Cell Biol."},{"key":"2024022708241815600_JOCES_107_10_2669C22","doi-asserted-by":"publisher","first-page":"127","DOI":"10.3109\/10520298409113845","article-title":"Polyester wax embedding and sectioning technique for immunohistochemistry","volume":"59","author":"Kusakabe","year":"1984","journal-title":"Stain Technol."},{"key":"2024022708241815600_JOCES_107_10_2669C23","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1083\/jcb.123.6.1899","article-title":"The dynamic structure of the pericellular matrix on living cells","volume":"123","author":"Lee","year":"1993","journal-title":"J. Cell Biol."},{"key":"2024022708241815600_JOCES_107_10_2669C24","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1084\/jem.175.1.257","article-title":"Requirements for hyaluronic acid binding by CD44: A role for the cytoplasmic domain and activation by antibody","volume":"175","author":"Lesley","year":"1992","journal-title":"J. Exp. Med."},{"key":"2024022708241815600_JOCES_107_10_2669C25","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/0167-4781(90)90018-W","article-title":"Effect of 1, 25(OH)2D3on its receptor mRNA levels and osteocalcin synthesis in human osteosarcoma cells","volume":"1048","author":"Mahonen","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"2024022708241815600_JOCES_107_10_2669C26","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.2106\/00004623-199173070-00017","article-title":"Characterization of aggregating proteoglycans from the proliferative, maturing, hypertrophic, and calcifying zones of the cartilagenous physis","volume":"73-A","author":"Matsui","year":"1991","journal-title":"J. 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Cell Biol."},{"key":"2024022708241815600_JOCES_107_10_2669C36","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1111\/1523-1747.ep12462776","article-title":"Migration of human melanoma cells on hyaluronate is related to CD44 expression","volume":"100","author":"Thomas","year":"1993","journal-title":"J. Invest. Dermatol."},{"key":"2024022708241815600_JOCES_107_10_2669C37","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/978-1-4613-0881-2_10","article-title":"Glycosaminoglycans in morphogenesis","volume-title":"Cell Biology of Extracellular Matrix","author":"Toole","year":"1981"},{"key":"2024022708241815600_JOCES_107_10_2669C38","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1242\/jcs.103.2.293","article-title":"CD44: The hyaluronan receptor","volume":"103","author":"Underhill","year":"1992","journal-title":"J. Cell Sci."},{"key":"2024022708241815600_JOCES_107_10_2669C39","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1111\/1523-1747.ep12371701","article-title":"Hyaluronan is inversely correlated with the expression of CD44 in the dermal condensation of the embryonic hair follicle","volume":"101","author":"Underhill","year":"1993","journal-title":"J. Invest. Dermatol."},{"key":"2024022708241815600_JOCES_107_10_2669C40","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1006\/dbio.1993.1032","article-title":"CD44 positive macrophages take up hyaluronan during lung development","volume":"155","author":"Underhill","year":"1993","journal-title":"Dev. 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