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We report that heparin is a trophic factor in primary cultures of rat small intestinal epithelium. Heparin elicits a dose-dependent increase in epithelial proliferation and inhibits the growth of associated mesenchyme. The trophic effect of this molecule is not reproduced by other glycosaminogly-cans including heparan sulphate but is dependent upon extensive molecular sulphation. Highly sulphated polysac-charides that are structurally unrelated to heparin (e.g. dextran sulphate and pentosan polysulphate) also stimulate epithelial proliferation in primary cultures. Heparin may act by the potentiation of mesenchyme-derived heparin-binding growth factors and these data suggest an in vivo role for mast cell-derived heparin in mucosal wound regeneration.<\/jats:p>","DOI":"10.1242\/jcs.107.2.401","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T11:55:02Z","timestamp":1619351702000},"page":"401-411","source":"Crossref","is-referenced-by-count":29,"title":["Heparin stimulates the proliferation of intestinal epithelial cells in primary culture"],"prefix":"10.1242","volume":"107","author":[{"given":"N.","family":"Flint","sequence":"first","affiliation":[{"name":"Cancer Research Campaign, Department of Epithelial Biology, Paterson Institute for Cancer Research, Wilmslow Road, Withington, Manchester M20 9BX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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